Investigation of photo-induced electron transfer between amino-functionalized graphene quantum dots and selenium nanoparticle and it's application for sensitive fluorescent detection of copper ions

Publication date: 15 May 2019

Source: Talanta, Volume 197

Author(s): Yi Li, Weilu Zhang, Xiangmei Jiang, Yanxia Kou, Jiajia Lu, Liang Tan

Abstract

Copper ions play an essential role in some biological processes. Currently, there is a need for the development of convenient and reliable analytical methods for the Cu²⁺ measurement. In the present work, a sensitive fluorescence method was developed for the determination of copper ions. Amino-functionalized graphene quantum dots (af-GQDs) and selenium nanoparticles (Se NPs) were synthetized, respectively, and they were characterized via transmission electron microscope, infrared spectrum analysis and X-ray photoelectron spectrum measurement. Photo-induced electron transfer (PET) between the prepared two nanomaterials could effectively quench the fluorescence of af-GQDs. Cu(II) was reduced to Cu(I) in the presence ascorbic acid and Cu₂Se was finally generated on Se NPs surface, which led to the declined PET efficiency and inhibited the fluorescence quenching of af-GQDs. The change in fluorescence intensity was linearly correlated to the logarithm of the Cu²⁺ concentration from 1 nM to 10 μM, with a detection limit of 0.4 nM under the optimal conditions. The detections of copper ions in water samples were realized via standard addition method and the recovery values varied from 98.7% to 103%. The proposed fluorescence method was also employed to analyze the uptake of Cu²⁺ into human cervical carcinoma HeLa cells and cisplatin-resistant HeLa cells (HeLa/DDP cells). The experimental results indicate that the decreased hCTR1 expression level in HeLa/DDP cells weakened the uptake of copper ions into these drug-resistant tumor cells.

Graphical abstract

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