Incretin-induced changes in the transcriptome of skeletal muscles of fa/fa Zucker rat (ZFR) with obesity, without diabetes

paythelady.61 shared this article with you from Inoreader Incretin-induced changes in the transcriptome of skeletal muscles of <i>fa/fa</i> Zucker rat (ZFR) with obesity, without diabetes Via International Journal of Obesity - Issue - nature.com science feeds View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

The prognostic value of adding systemic inflammation response index to Epstein–Barr virus DNA in childhood nasopharyngeal carcinoma: A real‐world study

paythelady.61 shared this article with you from Inoreader The prognostic value of adding systemic inflammation response index to Epstein–Barr virus DNA in childhood nasopharyngeal carcinoma: A real‐world study Via Head & Neck Abstract Background To assess the prognostic value of the systemic inflammation response index (SIRI) combined with plasma load of Epstein–Barr virus (EBV) DNA in children and adolescents with locoregionally advanced nasopharyngeal carcinoma (CALANPC). Methods A total of 205 consecutive patients with CALANPC were enrolled. We used recursive partitioning analysis (RPA) to classify patients into various risk groups, with a primary endpoint of overall survival (OS). Results Elevated SIRI (≥1.53) and EBV DNA (≥4000 copy/ml) were significantly associated with inferior OS in CALANPC. RPA categorized patients into low- and high-risk groups based on prognostic factors. Survival curves showed excellent discrimination in OS (95.3% vs 77.6%; p < 0.001) between the low- and high-risk groups. A significant improvement was confirmed using the prognostic methods for conventional TNM staging systems (p < 0.05). Conclusions The combination of SIRI with EBV DNA provided a more detailed understanding of patient risks, and enhanced risk discrimination in CALANPC. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Quantitative proteomics in medication‐related osteonecrosis of the jaw: a proof‐of‐concept study

paythelady.61 shared this article with you from Inoreader Quantitative proteomics in medication‐related osteonecrosis of the jaw: a proof‐of‐concept study Via Oral Diseases ABSTRACT Objective Medication-related osteonecrosis of the jaw (MRONJ) is a paradoxical effect associated with bone modifying agents (BMAs) and other drugs. Currently no valuable diagnostic or prognosis biomarkers exist. This goal of this research was to study MRONJ related salivary proteome. Materials and Methods This case-control aimed to study salivary proteome in MRONJ versus control groups i) formed from BMAs consumers and ii) healthy individuals to unravel biomarkers. 38 samples of unstimulated whole saliva (18 MRONJ patients, 10 BMA consumers, and 10 healthy controls) were collected. Proteomic analysis by SWATH-MS coupled to bioinformatics analysis was executed. Results 586 proteins were identified, 175 proteins showed significant differences among MRONJ versus controls. SWATH-MS revealed differentially expressed proteins among three groups, which have never isolated. These proteins had distinct roles including cell envelope organization, positive regulation of vesicle fusion, positive regulation of receptor binding, or regulation of low-density lipoprotein particle clearance. Integrative analysis prioritised 3 proteins (MMP9, AACT and HBD). Under receiver operating characteristic analysis, this panel discriminated MRONJ with a sensitivity of 90% and a specificity of 78.9%. Conclusion These findings may inform of a novel biomarker panel for MRONJ prediction or diagnosis. Nonetheless, further research is needed to validate this panel. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

N6‐methyladenosine modification contributes to arecoline‐mediated oral submucosal fibrosis

paythelady.61 shared this article with you from Inoreader N6‐methyladenosine modification contributes to arecoline‐mediated oral submucosal fibrosis Via Journal of Oral Pathology & Medicine Abstract Background Oral submucosal fibrosis (OSF) is a precancerous condition that closely related to the habit of chewing betel nut. The OSF patients of 3%–19% may develop cancer, and this probability is increasing year by year. Epigenetics modifications have been reported as part of the pathogenesis of OSF. However, in OSF field, the role and mechanism of arecoline-induced activation of transforming growth factor β (TGF-β) signaling on N6-methyladenosine (m6A) modification remain unclear. In this study, we investigated the effect and mechanism of arecoline on m6A modification. Methods MeRIP-Seq and RNA-seq were performed in arecoline-stimulated cells. Quantitative polymerase chain reaction and western blot were performed to detect the expression of m6A writers and erasers. CCK-8 and flow cytometry analyses were performed to measure the cell viability and apoptosis. Results m6A level was increased in OSF tissues compared to normal tissues; arecoline promoted the m6A methyltransferase Mettl3 and Mettl14 through TGF-β. MeRIP-seq and RNA-seq analyses found that MYC was the target gene of Mettl14. In addition, Mettl14 silence reversed the effects of arecoline on cell proliferation and apoptosis in Hacat cells. Conclusion TGF-β-METTL14-m6A-MYC axis was crucially implicated in arecoline-mediated OSF and may be an effective therapeutic strategy for OSF treatment. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Coverage error of three resin composite systems to vital unrestored maxillary anterior teeth

paythelady.61 shared this article with you from Inoreader Coverage error of three resin composite systems to vital unrestored maxillary anterior teeth Via Journal of Esthetic and Restorative Dentistry Abstract Objective Define the color of anterior teeth of a selected population and correlate it (by using coverage error [CE] and the frequency of best match) with the final color of all possible enamel-dentine combinations of three different resin composite systems. Materials and methods Color of 636 vital unrestored anterior teeth (central incisors, lateral incisors and canines; n = 212) and disk specimens (12 mm diameter, varying thickness) corresponding to enamel-dentin combinations of all available enamel (0.5 mm and 1.0 mm thickness) and dentin shades (3.0 mm thickness) of Essentia, Enamel Plus HRi and IPS Empress Direct composite systems was measured using a clinical dental spectrophotometer (Spectroshade Micro). CE and frequency of best match for all composite systems were calculated for the measured in-vivo teeth color space. Results Natural in-vivo teeth exhibit higher lightness when compared to enamel-dentin composite combinations, independently of the enamel thickness used. The best (lowest) CE was found for IPS Empress, while the highest values were found for Enamel Plus Hri independently of tooth type and enamel thickness (p < 0.001). The use of 0.5 mm instead of 1.0 mm enamel thickness within enamel-dentin composite combinations resulted in a lower CE for in-vivo tooth color (p < 0.001). Conclusions The color space defined by all possible enamel-dentin combinations of the studied resin composite systems does not fully match the color range of anterior teeth. All composite systems examined lack combinations with lightness values as high as the population's. IPS Empress Direct composite system represented better the in-vivo teeth color. Clinical significance The use of 0.5 mm enamel shade thickness is suggested when building layered restorations, as it provided better color coverage than using 1.0 mm thickness. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Applications of maxillary tuberosity block autograft

paythelady.61 shared this article with you from Inoreader Applications of maxillary tuberosity block autograft Via Journal of Esthetic and Restorative Dentistry Abstract Objective Autogenous bone grafts are considered the gold standard due to their compatibility and osteogenic potential to induce new bone formation through osteogenesis, osteoinduction, and osteoconduction. The aim of this paper was to describe clinical applications of the maxillary tuberosity block autograft in small and moderate localized defects of the alveolar process around implants and teeth. Clinical Considerations Maxillary tuberosity is often used as a particulate graft for augmentation of deficient alveolar ridge or maxillary sinus prior to or simultaneously with implant insertion, but not as a bone block graft. The maxillary tuberosity block autograft may also provide a valuable bone source for challenging situations such as immediate implant placement into types II and III extraction sockets, treatment of horizontal and vertical bone defects with simultaneous implantation, reconstruction of circumferential defects around implants, and preservation of alveolar ridge. Conclusions The advantages of the maxillary tuberosity include intraoral corticocancellous autogenous graft with fewer intraoperative difficulties, no need for donor site restoration, less morbidity, and an excellent correction of localized alveolar ridge defects. Clinical Significance Within the limitations of the presented case reports, the use of maxillary tuberosity block autograft has shown to be successful in alveolar ridges augmentation that lack both width and height. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Comparison of Responses to DCN vs. VCN Stimulation in a Mouse Model of the Auditory Brainstem Implant (ABI)

paythelady.61 shared this article with you from Inoreader Comparison of Responses to DCN vs. VCN Stimulation in a Mouse Model of the Auditory Brainstem Implant (ABI) Via Latest Results for Journal of the Association for Research in Otolaryngology Abstract The auditory brainstem implant (ABI) is an auditory neuroprosthesis that provides hearing to deaf patients by electrically stimulating the cochlear nucleus (CN) of the brainstem. Whether such stimulation activates one or the other of the CN's two major subdivisions is not known. Here, we demonstrate clear response differences from the stimulation of the dorsal (D) vs. ventral (V) subdivisions of the CN in a mouse model of the ABI with a surface-stimulating electrode array. For the DCN, low levels of stimulation evoked multiunit responses in the inferior colliculus (IC) that were unimodally distributed with early latencies (avg. peak latency of 3.3 ms). However, high levels of stimulation evoked a bimodal distribution with the addition of a late latency response peak (avg. peak latency of 7.1 ms). For the VCN, in contrast, electrical stimulation elicited multiunit responses that were usually unimodal and had a latency similar to the DCN's late respon se. Local field potentials (LFP) from the IC showed components that correlated with early and late multiunit responses. Surgical cuts to sever the output of the DCN, the dorsal acoustic stria (DAS), gave insight into the origin of these early and late responses. Cuts eliminated early responses but had little-to-no effect on late responses. The early responses thus originate from cells that project through the DAS, such as DCN's pyramidal and giant cells. Late responses likely arise from the spread of stimulation from a DCN-placed electrode array to the VCN and could originate in bushy and/or stellate cells. In human ABI users, the spread of stimulation in the CN may result in abnormal response patterns that could hinder performance. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.