Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria

Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria

Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria, Published online: 18 January 2019; doi:10.1038/s41467-018-08177-8

Gain of function methods based on gene overexpression are not easily applied to high-throughput screening across different experimental conditions. Here, the authors present Dub-seq, which separates overexpression library characterization from functional screening and uses random DNA barcodes to increase the experimental throughput.

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