Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics

Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics

Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics, Published online: 18 January 2019; doi:10.1038/s41467-018-08191-w

Spatial proteomics allows studying cellular protein localisations at system-wide scale. Here, the authors show that combining the previously developed hyperLOPIT method with differential centrifugation yields protein localisation maps at suborganellar resolution while reducing analysis time and input material.

from A via a.sfakia on Inoreader https://go.nature.com/2U01oTU