Low expression of miR-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of FAP.

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Low expression of miR-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of FAP.

Biosci Rep. 2017 Oct 12;:

Authors: Ruan P, Tan A, Tao Z

Abstract
The study aimed at investigating the effects of miR-30a-5p on the biological functions of oral cancer cells and figuring out the potential mechanism. We firstly verified the low expression of miR-30a-5p and high expression of FAP (Homo sapiens fibroblast activation protein alpha) in oral cancerous tissues and their negative correlation. Then, the target relationship between miR-30a-5p and FAP was validated by dual luciferase reporter assay and biotin-coupled miRNA pulldown assay. After transfection in Tca-8113 cells and SCC-15 cells, MTT, colony formation, Transwell and wound healing assays were performed to investigate how miR-30a-5p and FAP adjusted propagation, invasiveness and migration respectively. Mounting evidences supported that MiR-30a-5p directly targeted at FAP and suppressed its expression in oral cavity cancer cells (OSCCs). By suppressing FAP expression, miR-30a-5p significantly inhibited cell propagation, migration and invasion. Therefore, miR-30a-5p might be a new therapeutic target for oral cancer treatment.

PMID: 29026005 [PubMed - as supplied by publisher]

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