Effect of pore size on performance of monolithic tube chromatography of large biomolecules.

Effect of pore size on performance of monolithic tube chromatography of large biomolecules.

Electrophoresis. 2017 Sep 05;:

Authors: Podgornik A, Hamachi M, Isakari Y, Yoshimoto N, Yamamoto S

Abstract
Effect of pore size on the performance of ion-exchange monolith tube chromatography of large biomolecules was investigated. Radial flow 1 mL polymer based monolith tubes of different pore sizes (1.5, 2 and 6 μm) were tested with model samples such as 20mer poly T DNA, basic proteins and acidic proteins (molecular weight 14000-670000). Pressure drop, pH transient, the number of binding site, dynamic binding capacity and peak width were examined. Pressure drop-flow rate curves and dynamic binding capacity values were well correlated with the nominal pore size. While duration of the pH transient curves depends on the pore size, it was found that pH duration normalized on estimated surface area was constant, indicating that the ligand density is the same. This was also confirmed by the constant number of binding site values being independent on pore size. The peak width values were similar to those for axial flow monolith chromatography. These results showed that it is easy to scale up axial flow monolith chromatography to radial flow monolith tube chromatography by choosing the right pore size in terms of the pressure drop and capacity. This article is protected by copyright. All rights reserved.

PMID: 28873229 [PubMed - as supplied by publisher]

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