Metabolomics of primary cutaneous melanoma and matched adjacent extratumoral microenvironment

als.fakia shared this article with you from Inoreader Metabolomics of primary cutaneous melanoma and matched adjacent extratumoral microenvironment Via PLOS ONE Alerts: New Articles by Nicholas J. Taylor, Irina Gaynanova, Steven A. Eschrich, Eric A. Welsh, Timothy J. Garrett, Chris Beecher, Ritin Sharma, John M. Koomen, Keiran S. M. Smalley, Jane L. Messina, Peter A. Kanetsky Background Melanoma causes the vast majority of deaths attributable to skin cancer, largely due to its propensity for metastasis. To date, few studies have examined molecular changes between primary cutaneous melanoma and adjacent putatively normal skin. To broaden temporal inferences related to initiation of disease, we performed a metabolomics investigation of primary melanoma and matched extratumoral microenvironment (EM) tissues; and, to make inferences about progressive disease, we also compared unmatched metastatic melanoma tissues to EM tissues. Methods Ultra-high performance liquid chromatography—mass spectrometry-based metabolic profiling was performed on frozen human tissues. Results We observed 824 metabolites as differentially abundant among 33 matched tissue samples, and 1,118 metabolites as differentially abundant between metastatic melanoma (n = 46) and EM (n = 34) after false discovery rate (FDR) adjustment (p Conclusions Overall, pathway-bas ed results significantly distinguished melanoma tissues from EM in the metabolism of: ascorbate and aldarate, propanoate, tryptophan, histidine, and pyrimidine. Within pathways, the majority of individual metabolite abundances observed in comparisons of primary melanoma vs. EM and metastatic melanoma vs. EM were directionally consistent. This observed concordance suggests most identified compounds are implicated in the initiation or maintenance of melanoma. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.