The Effect of the Application Sequence of Casein Phosphopeptide‐Amorphous Calcium Phosphate Paste on the Shear Bond Strength of Etch‐and‐Rinse Resin Cements to Dentin

Abstract

Purpose

To evaluate the effect of the application sequence of casein phosphopeptide‐amorphous calcium phosphate (CPP‐ACP) paste (MI paste) on shear bond strength (SBS) of 2 etch‐and‐rinse resin cements to dentin.

Materials and Methods

Sixty extracted human maxillary premolars were cut to expose the mid‐coronal dentin surfaces. The teeth were randomly divided into 6 groups (n = 10), based on the resin cement used and CPP‐ACP application. In groups 1‒3, Excite DSC/Variolink II and in groups 2‒4, One‐Step Plus/Duolink was bonded to dentin. There were 3 groups for each cement as follows: with no treatment as a control; 3‐minute application of CPP‐ACP before acid etching (CPP‐ACP/Etching); 3‐minute application of CPP‐ACP after acid etching (Etching/CPP‐ACP). After storage in distilled water at 37°C for 24 hours, SBS test was performed. Data were analyzed with 2‐way ANOVA and post‐hoc Tukey test (α = 0.05).

Results

CPP‐ACP application on the dentin before and after acid etching significantly decreased the SBS of OS/Duolink (13.60 and 14.71 MPa, respectively vs. control; 21.21 MPa, p < 0.001); however, it did not influence the SBS of Excite DSC/Variolink II (25.14 MPa) before acid‐etching (27.37 MPa) or after acid etching (26.41 MPa) (p>0.388). The application sequence of CPP‐ACP did not influence the SBS of etch‐and‐rinse resin cements evaluated.

Conclusion

CPP‐ACP applications may lower the SBS in some etch‐and‐rinse resin cements, and timing of applications makes no difference.

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