SERCA directs cell migration and branching across species and germ layers [RESEARCH ARTICLE]

Danielle V. Bower, Nick Lansdale, Sonia Navarro, Thai V. Truong, Dan J. Bower, Neil C. Featherstone, Marilyn G. Connell, Denise Al Alam, Mark R. Frey, Le A. Trinh, G. Esteban Fernandez, David Warburton, Scott E. Fraser, Daimark Bennett, and Edwin C. Jesudason

Branching morphogenesis underlies organogenesis in vertebrates and invertebrates, yet is incompletely understood. Here, we show that the sarco-endoplasmic reticulum Ca²⁺ reuptake pump (SERCA) directs budding across germ layers and species. Clonal knockdown demonstrated a cell-autonomous role for SERCA in Drosophila air sac budding. Live imaging of Drosophila tracheogenesis revealed elevated Ca²⁺ levels in migratory tip cells as they form branches. SERCA blockade abolished this Ca²⁺ differential, aborting both cell migration and new branching. Activating protein kinase C (PKC) rescued Ca²⁺ in tip cells and restored cell migration and branching. Likewise, inhibiting SERCA abolished mammalian epithelial budding, PKC activation rescued budding, while morphogens did not. Mesoderm (zebrafish angiogenesis) and ectoderm (Drosophila nervous system) behaved similarly, suggesting a conserved requirement for cell-autonomous Ca²⁺ signaling, established by SERCA, in iterative budding.

from # All Medicine by Alexandros G. Sfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2hIyF4b
via IFTTT