Biosensors, Vol. 7, Pages 36: A Rationally Designed Reversible ‘Turn-Off’ Sensor for Glutathione

Biosensors, Vol. 7, Pages 36: A Rationally Designed Reversible 'Turn-Off' Sensor for Glutathione

Biosensors doi: 10.3390/bios7030036

Authors: Sabrina Heng Xiaozhou Zhang Jinxin Pei Andrew Abell

γ-Glutamyl-cysteinyl-glycine (GSH) plays a critical role in maintaining redox homeostasis in biological systems and a decrease in its cellular levels is associated with diseases. Existing fluorescence-based chemosensors for GSH acts as irreversible reaction-based probes that exhibit a maximum fluorescence ('turn-on') once the reaction is complete, regardless of the actual concentration of GSH. A reversible, reaction-based 'turn-off' probe (1) is reported here to sense the decreasing levels of GSH, a situation known to occur at the onset of various diseases. The more fluorescent merocyanine (MC) isomer of 1 exists in aqueous solution and this reacts with GSH to induce formation of the ring-closed spiropyran (SP) isomer, with a measurable decrease in absorbance and fluorescence ('turn-off'). Sensor 1 has good aqueous solubility and shows an excellent selectivity for GSH over other biologically relevant metal ions and aminothiol analytes. The sensor permeates HEK 293 cells and an increase in fluorescence is observed on adding buthionine sulfoximine, an inhibitor of GSH synthesis.

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