Single cell-based automated quantification of therapy response of invasive cancer spheroids in organotypic 3D culture

Publication date: Available online 22 July 2017 Source:Methods Author(s): Cornelia Veelken, Gert-Jan Bakker, David Drell, Peter Friedl Organotypic in vitro culture of 3D spheroids in extracellular matrix represent a promising cancer therapy prediction model for personalized medicine screens, due to their controlled experimental conditions and physiological similarities to in vivo models. As in tumors in vivo, 3D invasion cultures identify intratumor heterogeneity of growth, invasion and apoptosis induction by cytotoxic therapy. We here combine in vitro 3D spheroid invasion culture with irradiation and automated nucleus-based segmentation for single cell analysis to quantify growth, survival, apoptosis and invasion response during experimental radiation therapy. As output, multi-parameter ...

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