Transplanting GABAergic Neurons Differentiated from NSCs into Hippocampus Inhibits Seizures and Epileptiform Discharges in Pilocarpine-Induced Temporal Lobe Epilepsy Model.

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Transplanting GABAergic Neurons Differentiated from NSCs into Hippocampus Inhibits Seizures and Epileptiform Discharges in Pilocarpine-Induced Temporal Lobe Epilepsy Model.

World Neurosurg. 2019 Feb 18;:

Authors: Xu K, Liu F, Xu W, Liu J, Chen S, Wu G

Abstract
OBJECTIVE: This study aimed to explore whether intra-hippocampal transplantation of GABAergic neurons generated in vitro ameliorated seizures and epileptiform discharges via increasing GABA-associated inhibition mediated by the addition of new GABAergic neurons.
METHODS: NSCs isolated from newborn rats were induced and differentiated into GABAergic neurons. A total of 36 Pilocarpine-induced pharmacoresistant epileptic rats were divided into three groups: PBS group, NSCs group, and GABAergic neurons group (GABA group), with an additional ten normal rats used (NRC group). The effects of grafting on spontaneous recurrent seizures (SRS) were examined and hippocampal GABA content was measured after grafting.
RESULTS: In GABA group, the frequency of EEG decreased significantly compared to PBS group (P＜0.001), but there was no significant difference between GABA group and NSCs group. Compared with PBS group, the overall frequency, duration of SRS significantly decreased in transplantation group, especially in GABA group (P<0.01). The number of GABAergic neurons was the highest in the GABA group compared with the other groups (P＜0.001). Furthermore, hippocampal GABA concentrations significantly increased in the GABA group.
CONCLUSIONS: We demonstrate here that GABAergic neurons generated in vitro from NSCs and grafted into the hippocampi of chronically epileptic rats have the ability to significantly reduce the frequency of EEG, frequency and duration of SRS via increasing GABA-associated inhibition mediated by the addition of new GABAergic neurons.

PMID: 30790741 [PubMed - as supplied by publisher]

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