Identification and characterization of a highly metastatic epithelial cancer cell line from rat tongue cancer.

Related Articles

Identification and characterization of a highly metastatic epithelial cancer cell line from rat tongue cancer.

Arch Oral Biol. 2018 Nov;95:58-67

Authors: Qin X, Yan M, Li R, Ye D, Zhang J, Xu Q, Feng Y, Sun Q, Jiang C, Chen W

Abstract
OBJECTIVES: Tongue squamous cell carcinoma (TSCC) is a clinically devastating disease. However, most established TSCC cell lines currently show undesirable malignant behaviours. The purpose of this study is to establish a highly metastatic TSCC cell line to serve as a useful tool for basic research.
MATERIALS AND METHODS: TSCCs were induced by 4-nitroquinoline-1-oxide (4NQO) in Sprague-Dawley rats. Tumor cells were obtained from the cancer tissues by primary culture and were then purified by an in vitro invasion assay and a limiting dilution assay. The growth rate, cell cycle distribution, apoptotic rate, tumorigenicity and distant metastatic phenotypes of the rat tongue cancer cells were fully investigated and characterized.
RESULTS: To date, the rat tongue cancer cell line, named Rca-T, has been continuously cultured in vitro for over 210 passages and exhibit a long spindle-shaped morphology, adherent growth, and a stable epithelial phenotype. The population doubling time of Rca-T cells is 23.35 h. Approximately 39.8% of these cells are in S phase, and the apoptosis rate of Rca-T cells is 7.46%. Furthermore, in immunodeficient nude mice, both the xenograft rate and the incidence of experimental lung metastasis are 100%. The in vitro assays further reveal the highly malignant and epithelial-mesenchymal transition-like properties of Rca-T cells.
CONCLUSION: In this study, the tumorigenic and highly distant metastatic TSCC cell line Rca-T was established. The malignant features of this cell line, especially its metastatic potential, will enable a wealth of functional studies on the molecular mechanisms of TSCC metastasis in the future.

PMID: 30059808 [PubMed - indexed for MEDLINE]

from A via a.sfakia on Inoreader https://ift.tt/2ErpCRO