A ratiometric fluorescent probe for detection of endogenous and exogenous hydrogen sulfide in living cells

Publication date: 1 June 2019

Source: Talanta, Volume 198

Author(s): Ting Cao, Zhidong Teng, Deyan Gong, Jing Qian, Wei Liu, Kanwal Iqbal, Wenwu Qin, Huichen Guo

Abstract

A ratiometric visualized fluorescent probe of H₂S of intramolecular charge transfer (ICT) and excited intramolecular proton transfer (ESIPT) mechanisms due to solvation effects has been designed and synthesized. This chemosensor shows the distinct signal changes with dual-emission in blue and green fluorescence spectral channel (from 495 nm to 525 nm) upon the addition of H₂S in a single wavelength excitation. This chemosensor exhibits the low detection limit (91 nM) and high sensitivity and selectivity. Based on this, this chemosensor was successfully used not only to monitor H₂S exogenously but also used to detect and image the endogenously generated H₂S in HeLa cells with excellent performance.

Graphical abstract

from A via a.sfakia on Inoreader http://bit.ly/2TD34mr