One‐Pot Bioconversion of l‐Arabinose to l‐Ribulose in an Enzymatic Cascade

A spoonful of sugar: A new concept for the synthesis of l‐ribulose from l‐arabinose was successfully established using a one‐pot bioconversion consisting of engineered pyranose 2‐oxidase, xylose reductase, formate dehydrogenase, and catalase. Transient kinetics in combination with molecular dynamics simulations indicate that the sugar substrate is positioned closer to the FAD N5‐position in the engineered pyranose 2‐oxidase than in the wild‐type.

Abstract

This work reports the one‐pot enzymatic cascade that completely converts l‐arabinose to l‐ribulose using four reactions catalyzed by pyranose 2‐oxidase (P2O), xylose reductase, formate dehydrogenase, and catalase. As wild‐type P2O is specific for the oxidation of six‐carbon sugars, a pool of P2O variants was generated based on rational design to change the specificity of the enzyme towards the oxidation of l‐arabinose at the C2‐position. The variant T169G was identified as the best candidate, and this had an approximately 40‐fold higher rate constant for the flavin reduction (sugar oxidation) step, as compared to the wild‐type enzyme. Computational calculations using quantum mechanics/molecular mechanics (QM/MM) molecular dynamics (MD) showed that this improvement is due to a decrease in the steric effects at the axial C4‐OH of l‐arabinose, which allows a reduction in the distance between the C2‐H and flavin N5, facilitating hydride transfer and enabling flavin reduction.

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