Engineering of CRISPR-Cas12b for human genome editing
Engineering of CRISPR-Cas12b for human genome editing, Published online: 22 January 2019; doi:10.1038/s41467-018-08224-4
The Cas12b family of CRISPR nucleases has been underutilized in mammalian cells due to the high temperature requirement of known members. Here the authors engineer BhCas12b to overcome this limitation for robust and specific genome editing applications in human cells.
from A via a.sfakia on Inoreader https://go.nature.com/2sGQJ4M
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Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,