Construction of a novel far-red fluorescence light-up probe for visualizing intracellular peroxynitrite

Publication date: 15 May 2019

Source: Talanta, Volume 197

Author(s): Wangbo Qu, Changhe Niu, Xiaoyu Zhang, Wei Chen, Fabiao Yu, Heng Liu, Xiuhua Zhang, Shengfu Wang

Abstract

Peroxynitrite (ONOO^-) is an important reactive oxygen species (ROS), which can react with a variety of biologically active species and cause many diseases, such as cancer, neurodegenerative disorders. Herein, we develop a novel far-red fluorescent probe DCM-KA, which is equipping with α-ketoamide moiety caged DCM-NH₂. The probe exhibits fluorescence off-on response to ONOO^- over other biological interfering analytes by ONOO^--induced α-ketoamide deprotection reaction. More importantly, the probe is biocompatible and has been successfully utilized to visualize endogenous ONOO^- production in macrophage cell line J774A.1.

Graphical abstract

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