Bioassay-guided isolation of anti-seizure principles from Semen Pharbitidis using a zebrafish pentylenetetrazol seizure model.

Bioassay-guided isolation of anti-seizure principles from Semen Pharbitidis using a zebrafish pentylenetetrazol seizure model.

J Ethnopharmacol. 2018 Dec 17;:

Authors: Liu M, Copmans D, Lu JG, Yang MR, Sourbron J, Ny A, Jiang ZH, de Witte PAM, Luyten W

Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Semen Pharbitidis, the seeds of Pharbitis nil (Linn.) Choisy (Convolvulaceae) is a well-known traditional Chinese medicinal plant used for treating helminthiasis and epilepsy in China.
AIM OF THE STUDY: This study aims to identify the anti-seizure components from Semen Pharbitidis.
METHODS: A bioassay-guided isolation of anti-seizure compounds from Semen Pharbitidis was performed using a zebrafish pentylenetetrazol seizure model. The structures of active compounds were elucidated by high resolution mass spectrometry. The fragments of active compounds were tested for anti-seizure activity as well.
RESULTS: The bioassay-guided isolation of ethanol extract of Semen Pharbitidis led to a group of resin glucosides, namely pharbitin. One of the fragments of pharbitin, 2-methylbutyric acid, also showed anti-seizure activity.
CONCLUSIONS: We provided further experimental scientific evidence to support the traditional use of Semen Pharbitidis for the treatment of epilepsy. Pharbitin was identified to be the main anti-seizure component in Semen Pharbitidis.

PMID: 30572093 [PubMed - as supplied by publisher]

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