Periodontal treatment modulates gene expression of endothelial progenitor cells in diabetic patients

Abstract

Background

Periodontitis is closely linked with type 2 diabetes mellitus (T2DM) and endothelial dysfunction. This study investigated the effects of periodontal treatment on immuno-inflammatory gene expression of endothelial progenitor cells (EPCs) in diabetic patients.

Methods

Eighteen T2DM patients with moderate to severe chronic periodontitis were randomly assigned to the Treatment group with oral hygiene instruction (OHI), scaling and root debridement (n=11), and Control group (n=7) with OHI alone. Peripheral blood samples were taken for biochemical analysis and culture of EPCs at baseline and 6 months after the treatment. PCR array-based profiling of 84 Toll-like receptor signaling-related genes in EPCs was firstly assessed for four randomly selected patients from the Treatment group. The differentially expressed genes were then further validated by qPCR in both groups.

Results

All subjects in the Treatment group significantly improved their periodontal conditions. Among the 84 genes tested, IL-6 and IL-8 transcripts were significantly down-regulated with over 2-fold change after the treatment, and this observation was further validated by qPCR in all subjects from both groups (p<0.05).

Conclusion

This preliminary study suggests that periodontal treatment may contribute to a notable reduction of immuno-inflammatory gene expression measured by IL-6 and IL-8 transcripts in EPCs.

This article is protected by copyright. All rights reserved.

from ! ORL Sfakianakis via paythelady.61 on Inoreader http://ift.tt/2irXXHo
via IFTTT