GSE100193 Novel chimeric transcript RRM2-c2orf48 promotes metastasis in nasopharyngeal carcinoma

Contributors : Ping Han ; Ren-Hui Chen ; Fang Wang ; Jia-Yi Zeng ; Tian-Liang Xia ; Qian Zhong ; Xiao-Ming HuangSeries Type : Expression profiling by arrayOrganism : Homo sapiensA microarray analysis was performed on the vector and over-expressing RRM2-c2orf48 NPC cell samples(CNE2-PMSCV-Vector and CNE2-PMSCV-RRM2-c2orf48).Total RNA was extracted from 1 × 106 cells using TRIzol reagent and was further purified using a Qiagen RNeasy Mini Kit according to the manufactures' instructions. RNA quality was assessed by formaldehyde agarose gel electrophoresis and was quantitated spectrophotometrically. Total RNA was labeled and hybridized to the Capita lBio Corporation's Affymetrix GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix). We applied a two class unpaired method available throug...

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