Effect of fibulin-5 on aldosterone-induced apoptosis in human ascending aortic smooth muscle cells

xlomafota13 shared this article with you from Inoreader Effect of fibulin-5 on aldosterone-induced apoptosis in human ascending aortic smooth muscle cells Via Experimental and therapeutic medicine Exp Ther Med. 2021 Aug;22(2):896. doi: 10.3892/etm.2021.10328. Epub 2021 Jun 23. ABSTRACT The aim of the current study was to investigate the effect of aldosterone on apoptosis in human aortic smooth muscle cells (HA-VSMC) and to determine the role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC cells. Through the construction of a fibulin-5 eukaryotic overexpression vector and a short hairpin RNA interference plasmid, fibulin-5 was overexpressed and silenced, respectively. The role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC was subsequently determined. The overexpression of fibulin-5 inhibited the apoptosis of cells, particularly at low concentrations of aldosterone; a smaller effect on apoptosis was induced by high concentrations of aldosterone. fibulin-5 knockdown promoted the apoptosis of cells induced by high concentrations of aldosterone but had a smaller effect on the apoptosis of cells induced by low concentrations of aldosterone. Therefore, the results of the current study indicate that fibulin-5 inhibits the aldosterone-induced apoptosis of HA-VSMC cells and that this effect may be altered by changing the aldosterone concentration. PMID:34257709 | PMC:PMC8243313 | DOI:10.3892/etm.2021.10328 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.