New Recombinant Phytase from Kosakonia sacchari : Characteristics and Biotechnological Potential

xlomafota.13 shared this article with you from Inoreader New Recombinant Phytase from Kosakonia sacchari : Characteristics and Biotechnological Potential Via Latest Results for Applied Biochemistry and Microbiology Abstract A DNA sequence from Kosakonia sacchari that, according to automated computer analysis, is believed to correspond to the gene for histidine-acid phytase has been selected from the GenBank database. The sequence was optimized for codon composition, synthesized, cloned, and expressed in Pichia pastoris. The main characteristics of the purified recombinant enzyme were determined. It was established that a pH of 4.5 and a temperature of 50°C are optimal for phytase functioning. The specific activity, Michaelis constant (Km), and maximum reaction rate (Vmax) with phytate as a substrate were 1470 U/mg, 193 μM and 2167 μmol/(min mg), respectively. It was shown that the enzyme was characterized by a wide range of working pH levels. Therefore, due to its properties, this new recombinant phytase can be considered a high-potential enzyme for agrobiotechnology. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.