Expression of the NADPH + -Dependent Formate-Dehydrogenase Gene from Pseudomona s Increases Lysine Production in Corynebacterium glutamicum

xlomafota.13 shared this article with you from Inoreader Expression of the NADPH + -Dependent Formate-Dehydrogenase Gene from Pseudomona s Increases Lysine Production in Corynebacterium glutamicum Via Latest Results for Applied Biochemistry and Microbiology Abstract The psefdh_D221Q gene encodes a mutant formate dehydrogenase from Pseudomonas (P-seFDG_D221Q), which catalyzes formate oxidation with the simultaneous formation of NADPH. It is expressed in cells of lysine-producing Corynebacterium glutamicum strains. The psefdh_D221Q gene was introduced into C. glutamicum strains as part of an autonomous plasmid or was integrated into the chromosome with the simultaneous inactivation of the host formate-dehydrogenase genes. It was shown that the C. glutamicum strains with NADP+-dependent formate dehydrogenase have an increased level of L-lysine synthesis in the presence of formate if their own formate dehydrogenase is inactivated. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.