Brazilein induces apoptosis and G1/G0 phase cell cycle arrest by up-regulation of miR-133a in human vestibular schwannoma cells.

Brazilein induces apoptosis and G1/G0 phase cell cycle arrest by up-regulation of miR-133a in human vestibular schwannoma cells.

Exp Mol Pathol. 2019 Jan 02;:

Authors: Mou Z, Wang Y, Li Y

Abstract
BACKGROUND: Acoustic neuroma is a benign and usually slow growing tumor. Brazilein is a natural compound extracted from hematoxylin. However, there has been no study of the mechanism of brazilein in acoustic neuroma cells. Thus, we aimed to investigate the effects and mechanism of brazilein on human VS cells in this study.
METHODS: The vestibular schwannoma (VS) cells were collected from patient tissues and used in this study. Different concentrations of brazilein (0, 10, 20 and 30 μM) were used to treat VS cells. The expression of miR-133a was altered by transfection with miR-133a inhibitor. Further, cell viability, apoptosis, cell cycle, the mRNA and phosphorylation levels of cell cycle, apoptosis-related proteins and main factors in MAPK and JNK pathways were detected using CCK-8 assay, flow cytometry analysis, qRT-PCR and western blot analysis, respectively.
RESULTS: The results showed that brazilein decreased cell viability, increased apoptosis and induced G1/G0 cell cycle arrest in VS Cells. Further, miR-133a expression was up-regulated in the brazilein-treated cells. Brazilein promoted apoptosis and induced G1/G0 cell cycle arrest via up-regulation of miR-133a. In addition, brazilein inhibited the activations of MAPK and JNK pathways by up-regulating miR-133a expression.
CONCLUSION: In conclusion, our study demonstrated that brazilein could induce apoptosis and G1/G0 phase cell cycle arrest, and deactivate MAPK and JNK signaling pathways via up-regulation of miR-133a in human VS cells. These results provide theoretical evidence for the clinical application of brazilein and a new strategy for the treatment of acoustic neuroma.

PMID: 30610843 [PubMed - as supplied by publisher]

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