Anti-cancer effects of CQBTO, a chloroquine and benzo(e)triazine oxide conjugate.

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Anti-cancer effects of CQBTO, a chloroquine and benzo(e)triazine oxide conjugate.

Chem Biol Drug Des. 2019 Jan 13;:

Authors: Guo Z, Pei H, Nie J, Hu W, Zhang J, Ding J, Pan S, Li B, Hei TK, Chen W, Zhou G

Abstract
AIM: Autophagy is a self-protective process, and it confers cancer cells resistance against radio-chemotherapeutics. To induce cancer cell death, a series of compounds of 3- ((4-((7-chloroquinolin-4-yl)amino)butyl)amino)- 7-substituted benzo[e][1,2,4] triazine 1-oxide or CQBTO containing two critical chemical groups were designed and synthesized. One compound, BTO, yielded free radicals to trigger autophagy, and the other one, chloroquine (CQ), was an inhibitor of autophagy. We hypothesized that the compounds could kill cancer cells effectively by inducing incomplete autophagy.
METHODS: In vitro cultured non-small cell lung carcinoma cells and primary lung tumors in mice in vivo were used to test the lethal effects of CQBTO on cancer cells and toxicity to normal tissues. Cell viability was examined using the CCK8 assay. Genomic instability was determined with the cytochalasin B-blocked micronucleus assay. Cell cycle distribution was analyzed by propidium iodide staining and flow cytometry. Western blotting and immunofluorescence were used to detect the induction and localization of LC3, a biomarker for autophagy.
RESULTS: Compared with CQ, three CQBTO compounds were lethal to lung cancer cells, and CQBTO-3 was the most effective. The LD50 for CQBTO-3 was 21 μΜ in A549 cells and 21.5 μΜ in Calu-1 cells, which was lower than that of CQBTO-2 or CQBTO-1. Induction of LC3 foci and an increase in the LC3II/LC3I ratio demonstrated the induction of autophagy by CQBTO-3 in A549 cells, whereas no obvious micronuclei or cell cycle arrest was observed. No detectable toxicity to normal mice was observed. CQBTO-3 improved the quality of mouse life, reduced the number and size of existing tumors, and suppressed tumor formation.
CONCLUSION: CQBTO-3 is a potential chemical compound for lung cancer treatment. This article is protected by copyright. All rights reserved.

PMID: 30637976 [PubMed - as supplied by publisher]

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