A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus

Publication date: 15 May 2019

Source: Talanta, Volume 197

Author(s): Caroline R. Basso, Bruno P. Crulhas, Massimiliano Magro, Fabio Vianello, Valber A. Pedrosa

Abstract

A new immunosensor using hybrid nanomaterials for the detection of dengue virus was demonstrated in this work. This immunosensor composed of nanoparticles of γ-Fe₂O₃(SAMN) modified with MPA- SAMN@MPA was characterized by FTIR spectroscopy, transmission electron microscopy,quartz crystal microbalance, UV–vis and LSPR technique. The binding of SAMN@MPA with AuNPs conjugated with aptamers(SAMN@MPA@AuNPs@aptamer) provides specific chemical bonds to four dengue serotypes. Colorimetric changes in the modification steps provided rapid visual detection of the virus without the use of equipment. Variations of aptamers concentrations 1.0-10.0 μM where the 3.0 μM aptamer concentration is sufficient to completely cover the surface of the modified AuNPs with an R² value of> 0.99. This new proposed methodology presenting some advantages in relation to traditional detection methods such as time optimization and cost,can be used as a diagnostic method.

Graphical abstract

The colorimetric tests show SAMNs (A), AuNPs (B), SAMNs@MPA@AuNPs@aptamer complex (C), after addition of the DENV pool (D), interference test with ZIKV (E) and YFD (F).

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