Αρχειοθήκη ιστολογίου

Κυριακή 10 Σεπτεμβρίου 2017

Extraction, characterization and bioactive properties of Nigella sativa seedcake

Abstract

The extraction of phenolic compounds from Nigella sativa seed cake was optimized in terms of % of EtOH, extraction time, extraction temperature and solid to solvent ratio to maximize the phenolic content yield. The optimized conditions were 40% ethanol for 60 min, at 40 °C, 1/14 solid to sample ratio. The LC–MS profiling of the extract was found to contain many important phenolics such as Kaempferol, p-coumaroyl acid derivative, Thymol-O-sophoroside etc. The extract showed significant antioxidant activity with IC50 values 548.5 ± 9.4, 64.3 ± 2.7 μg/ml and 1.85 ± 0.2 mM TE/g in terms of DPPH scavenging activity, TEAC, and FRAP assay respectively. The results also indicated that the extract has a significant anti-inflammatory potential. This was evaluated as a measure of the membrane stabilization ability and protein denaturation inhibition capacity (IC50 values) and the activities were found to be 318.73 ± 6.98 and 150.39 ± 2.61 μg/ml respectively. Moreover, results of the study are promising and invite to further investigate the above activities in order to confirm them in different experimental situations and to consider for possible use in a nutraceutical approach.



from # All Medicine by Alexandros G. Sfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2vOxh5Y
via IFTTT

Δεν υπάρχουν σχόλια:

Δημοσίευση σχολίου

Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,

Αναζήτηση αυτού του ιστολογίου

! # Ola via Alexandros G.Sfakianakis on Inoreader