Phase-contrast hard X-ray microscopy using synchrotron radiation for the properties of skeletal muscle in mouse hind limbs

Abstract

Radiation beam interface contrast X-ray microscopy provides resolution of a few dozen nanometers from fixed whole muscle biopsies, allowing better reconstruction of the microstructure of the muscle than is currently possible with classic histological techniques. Fixed soleus muscle biopsies have been evaluated from the walk-in mouse model using phase-contrast X-ray microscopy, and results presented that corroborate the accuracy of the method used, and its potential for application in physiotherapy and occupational therapy studies. We believe that this method will enhance existing morphometric methods of analysis, leading to accurate reconstruction of other thick specimens that would otherwise require thin sectioning and reconstruction through deconvolution algorithms.

A new method is suggested that can observe the soleus muscle by means of a radiation beam. This study suggests a remarkable method for analyzing muscle tissue that provides a simple and unified technique for obtaining stereoscopic tissue images.

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