Towards Sensitive, High-Throughput, Biomolecular Assays Based on Fluorescence Lifetime.
Methods Appl Fluoresc. 2017 Jul 12;:
Authors: Skilitsi AI, Turko T, Cianfarani D, Barre S, Uhring W, Hassiepen U, Léonard J
Abstract
Time-resolved fluorescence detection for robust sensing of biomolecular interactions is developed by implementing Time Correlated Single Photon Counting in high-throughput conditions. Droplet microfluidics is used as a promising platform for the very fast handling of low-volume samples. We illustrate the potential of this very sensitive and cost-effective technology in the context of an enzymatic activity assay based on fluorescently-labeled biomolecules. Fluorescence lifetime detection by Time Correlated Single Photon Counting is shown to enable reliable discrimination between positive and negative control samples at a throughput as high as several hundred samples per second.
PMID: 28699919 [PubMed - as supplied by publisher]
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