Resveratrol attenuates excessive ethanol exposure induced insulin resistance in rats via improving NAD(+) /NADH ratio.

Resveratrol attenuates excessive ethanol exposure induced insulin resistance in rats via improving NAD(+) /NADH ratio.

Mol Nutr Food Res. 2017 Jul 08;:

Authors: Luo G, Huang B, Qiu X, Xiao L, Wang N, Gao Q, Yang W, Hao L

Abstract
SCOPE: Resveratrol has been shown to improve insulin resistance via activating the NAD(+) -dependent deacetylase SIRT1, but the effects of resveratrol on ethanol-induced insulin resistance remain unclear. This study was designed to explore the potential mechanism by which resveratrol ameliorated ethanol-induced insulin resistance, focusing on its regulations on the ratio of NAD(+) /NADH and SIRT1 expression.
METHODS AND RESULTS: Male Sprague-Dawley rats were fed either control or ethanol liquid diets containing 0.8, 1.6 and 2.4 g/kg·bw ethanol with or without 100 mg/kg·bw resveratrol for 22 weeks. Resveratrol improved ethanol (2.4 g/kg·bw) induced reductions in insulin sensitivity, SIRT1 expression (51%, P < 0.05), NAD(+) /NADH ratio (196%, P < 0.01) as well as the expression and activity of ALDH2 while decreased the augmentations in the expression and activity of ADH and CYP2E1. In primary rat hepatocytes, ethanol exposure (25 mmol/L, 24 hours) similarly decreased SIRT1 expression and NAD(+) /NADH ratio (33%, P < 0.05, 32%, P < 0.01), and 0.1 μmol/L resveratrol treatment reversed these decreases and inhibited the expressions of ADH and CYP2E1.
CONCLUSION: Resveratrol exhibits benefits against ethanol-induced insulin resistance via improving the ratio of NAD(+) /NADH to regulate SIRT1, which is associated with the modulation of ethanol metabolism enzymes. This article is protected by copyright. All rights reserved.

PMID: 28688179 [PubMed - as supplied by publisher]

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