Dual Fluorescence Nano-conjugates for Ratiometric Detection of Reactive Oxygen Species in Inflammatory Cells.

Dual Fluorescence Nano-conjugates for Ratiometric Detection of Reactive Oxygen Species in Inflammatory Cells.

J Biophotonics. 2017 Jul 12;:

Authors: Ma Y, Wang Q, Zhu Q, Liu M, Chen D, Han Z, Zhang C, Lv L, Gu Y

Abstract
Reactive oxygen species (ROS) are largely produced under pathological situations. To understand the etiology of disease, it is urgent to develop efficacious probes for detecting ROS. Herein, a novel nano-conjugate detection system constructed from gold clusters (AuNCs) and quantum dots (QDs) for fluorescence ratio metric sensing ROS was reported. Upon interacting with ROS, the red emission fluorescence (645 nm from QDs) in the detection system was gradually decreased, while the green fluorescence (480 nm from AuNCs) changed little. The fluorescence ratio at the two wavelengths (I480nm /I645nm ) was linear correlated with the ROS which could be used for the real time ratio metric detection of ROS. The developed nano-conjugates can be applied to monitor the ROS in inflammatory cells for its ability of generating abundant ROS and uptaking ability to nanopartilces. The stimulated ROS in inflammatory cells were monitored by AuNC-QD and the results were consistent with the traditional DCFH-DA method, confirming the reliability of the developed method. Featured with the merits of higher photostability, low background, high accuracy of ratiometric detection, the AuNC-QD conjugate demonstrated its potential to be the probe for realtime ROS detection in inflammatory cells.

PMID: 28700134 [PubMed - as supplied by publisher]

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