Optimization of a GF-AAS method for lead testing in blood and urine: A useful tool in acute abdominal pain management in emergency

xlomafota13 shared this article with you from Inoreader Optimization of a GF-AAS method for lead testing in blood and urine: A useful tool in acute abdominal pain management in emergency Via Experimental and therapeutic medicine Exp Ther Med. 2021 Sep;22(3):985. doi: 10.3892/etm.2021.10417. Epub 2021 Jul 13. ABSTRACT Suspicion of lead poisoning is confirmed by its concentration in blood and protoporphyrin red blood cells. At low concentrations, lead influences the synthesis of the heme in the sense of lowering it. Acute and chronic lead intoxication is extremely polymorphic in regards to its clinical manifestations, with digestive, hematological, cardiovascular, renal hepatic and neurological features. The aim of the study was to evaluate the presence of lead in human whole blood and urine harvested before and during chelation treatment in the case of lead poisoning. An atomic absorption spectroscopic method for the analysis of lead was developed using graphite furnace atomic absorption spectrophotometer (GF-AAS), Varian Spectra AA-880 with a hollow cathode lead lamp and a deuterium lamp for background correction, coupled to a GTA-100 atomizer and a programmab le sample dispenser. Standard calibration solutions were used for the range 10-100 µg/l. The linearity range was 10.0 to 100.0 µg/l with the correlation coefficient of 0.999. We established that the method can be applied for the determination of lead in whole blood and urine, and the results obtained are useful for monitoring chelation therapy in cases of acute lead poisoning, a neglected cause of abdominal colic pain in an emergency situation. PMID:34345267 | PMC:PMC8311247 | DOI:10.3892/etm.2021.10417 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.