miR-374a-5p inhibits non-small cell lung cancer cell proliferation and migration via targeting NCK1

xlomafota13 shared this article with you from Inoreader miR-374a-5p inhibits non-small cell lung cancer cell proliferation and migration via targeting NCK1 Via Experimental and therapeutic medicine Exp Ther Med. 2021 Sep;22(3):943. doi: 10.3892/etm.2021.10375. Epub 2021 Jul 1. ABSTRACT Emerging studies have indicated that microRNAs (miRNAs/miRs) are involved in regulating non-small cell lung cancer (NSCLC)-associated processes. The present study aimed to evaluate the biological roles of miR-374a-5p in NSCLC. Using reverse transcription-quantitative PCR, the expression levels of miR-374a-5p were determined in NSCLC cells and a normal cell line. Functional experiments were performed to investigate the functions of miR-374a-5p in NSCLC. A luciferase activity reporter assay and rescue experiments were performed to validate NCK adaptor protein 1 (NCK1) as a functional target of miR-374a-5p. It was demonstrated that miR-374a-5p levels were decreased in NSCLC cell lines compared with those in a normal cell line. Furthermore, overexpression of miR-374a-5p inhibited NSCLC cell proliferation and migration in vitro. Of note, NCK1 ove rexpression reversed the effects of miR-375a-5p on NSCLC cell proliferation and migration. The present results confirmed the tumor suppressor role of miR-374a-5p via targeting NCK1 in NSCLC, indicating the importance of the miR-374a-5p/NCK1 axis in NSCLC. PMID:34306207 | PMC:PMC8281440 | DOI:10.3892/etm.2021.10375 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.