GLP-1 mediated improvement of the glucose tolerance in the T2DM GK rat model after massive jejunal resection

Publication date: Available online 2 February 2019

Source: Annals of Anatomy - Anatomischer Anzeiger

Author(s): J. Arturo Prada-Oliveira, Alonso Camacho-Ramírez, Jesús Salas-Alvarez, Francisco Javier Campos-Martínez, Alfonso M. Lechuga-Sancho, David Almorza-Gomar, Manuel Blandino-Rosano, Gonzalo M. Pérez-Arana

Abstract

Objective

The aim of this study was to clarify the role of the middle gut in the entero-pancreatic axis modification that leads to glucose improvement in the Goto-Kakizaki (GK) rat as a non-obese T2DM model.

Background

Bariatric surgery is considered an assured solution for type 2 Diabetes (T2DM). Enterohormones such as ghrelin, gastric inhibitory polypeptide and mainly glucagon-like peptide-1 (GLP-1) were recognized as key players in the physiophathological mechanisms associated with entero-pancreatic axis regulation and glucose tolerance improvement. However, the influence of anatomical arrangements post-bariatric surgery on this axis is still debatable.

Method

To this purpose, 50% of small intestine resections were performed on GK rats (n = 6), preserving the proximal half of the jejunum and the ileum (IR50). Phenotypic and functional changes, such as performance in oral glucose tolerance tests, ileal release of GLP-1, beta-cell sensitivity to GLP-1, beta-cell mass, and turnover were characterized in IR50 and the surgical control group (Sham).

Results

The glucose tolerance was improved and ileal release of GLP-1 was enhanced four weeks after IR50 versus the control group rats. Beta-cell mass, beta-cell proliferation, and beta-cell sensitivity to GLP-1 were also increased in the pancreas of IR50 versus the control group rats.

Conclusion

the jejunal exclusion increases beta-cell-mass and improves glucose tolerance by increasing in GLP-1 expression and number of receptors via the entero-pancreatic axis.

from A via a.sfakia on Inoreader http://bit.ly/2S5I62F