A novel thermometer-type hydrogel senor for glutathione detection

Publication date: 1 May 2019

Source: Talanta, Volume 196

Author(s): Rui Wu, Hongguang Ge, Cunfang Liu, Shenghai Zhang, Liang Hao, Qiang Zhang, Juan Song, Guanghui Tian, Jiagen Lv

Abstract

A thermometer-type visual sensor for glutathione (GSH) sensing was developed with stimulus-responsive fluorescent hydrogel which was obtained by using 5, 6-bicarboxylic fluorescein crossli`nked partly ammoniated polyacrylamide. Various experimental parameters such as the particle size of hydrogel, buffer solution and swelling time were optimized. It is accessible to measure the volume change of hydrogel with the sensor by reading the graduation on a pipette like thermometer with naked eye. The concentration of the GSH depended on the volume in a certain range as the signal. Satisfactory agreements between the sensor and HPLC results for atuomolan tablet assays indicated the capability of the thermometer-type sensors for the analysis of real samples. These findings proved the utility of stimulus-responsive, intelligent hydrogel and the suitability of thermometer-style visual sensor design for quantitative assays.

Graphical abstract

5,6-dicarboxylic fluorescein (5,6-DCF) cross-linked amine-functionalized polyacrylamide (PAM) hydrogel for volumetric response to glutathione. Photographic images of the hydrogel sensor and its responses to glutathione which can be quantitatively distinguished like a thermometer-style by the naked eye.

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