miRNA Expression Profile of Mucoepidermoid Carcinoma

Abstract

Objectives

MicroRNAs (miRNAs) are single-stranded RNAs that have been implicated in cancer initiation and progression and act as tumour suppressors or oncogenes. In this study, miRNA profiling was conducted on the most frequent malignancy of salivary glands, mucoepidermoid carcinoma (MEC), with comparison to normal tissues.

Materials and Methods

The TaqMan Human miRNA Cards Array was used for the miRNA profiling of MEC and normal tissues. To validate the differentially expressed miRNAs in MEC, we used real-time PCR (qRT-PCR).

Results

miR-302a was the most significantly increased miRNA in cancer tissues (p < 0.05). Here, we demonstrate that the up-regulation of miR-302a expression in SGT cell lines induced cancer cell invasion in vitro.

Conclusions

These promising results suggest the need for further studies to establish mir-302a as a marker of invasion and aggressiveness in MEC.

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