Use of a Rapid Reverse-transcription Recombinase Aided Amplification Assay for Respiratory Syncytial Virus Detection

Publication date: Available online 14 October 2017
Source:Diagnostic Microbiology and Infectious Disease
Author(s): Chen Chen, Xin-na Li, Gui-xia Li, Li Zhao, Su-xia Duan, Teng-fei Yan, Zhi-shan Feng, Xue-jun Ma
In this study, a rapid reverse-transcription recombinase aided amplification (RT-RAA) assay was developed to detect respiratory syncytial virus (RSV) subgroups A and B, respectively. The reaction was performed at 39°Cin less than 30min. The analytical sensitivities of RSVA and RSVB at 95% probability by probit regression analysis were 38copies per reaction and 35 copies per reaction, respectively, and no cross reactions with other related respiratory viruses were observed. The RT-RAA assay was further utilized to detect and subgroup 306 clinical specimens and the results showed that 79(25.82%, 79/306) samples were positive for RSV, of those 16(20.25%, 16/79) were identified as RSVA and 63(79.75%, 63/79) were RSVB, which is completely consistent with the results obtained by RSV RT-qPCR assay. In conclusion, the developed RAA assay will be of benefit as a faster, sensitive and specific alternative tool for detection of RSV.



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