IJMS, Vol. 18, Pages 2210: Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine

IJMS, Vol. 18, Pages 2210: Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine

International Journal of Molecular Sciences doi: 10.3390/ijms18102210

Authors: Léa Aubert Marie Dubus Hassan Rammal Camille Bour Céline Mongaret Camille Boulagnon-Rombi Roselyne Garnotel Céline Schneider Rachid Rahouadj Cedric Laurent Sophie C. Gangloff Frédéric Velard Cedric Mauprivez Halima Kerdjoudj

Maintenance of mesenchymal stem cells (MSCs) requires a tissue-specific microenvironment (i.e., niche), which is poorly represented by the typical plastic substrate used for two-dimensional growth of MSCs in a tissue culture flask. The objective of this study was to address the potential use of collagen-based medical devices (HEMOCOLLAGENE®, Saint-Maur-des-Fossés, France) as mimetic niche for MSCs with the ability to preserve human MSC stemness in vitro. With a chemical composition similar to type I collagen, HEMOCOLLAGENE® foam presented a porous and interconnected structure (>90%) and a relative low elastic modulus of around 60 kPa. Biological studies revealed an apparently inert microenvironment of HEMOCOLLAGENE® foam, where 80% of cultured human MSCs remained viable, adopted a flattened morphology, and maintained their undifferentiated state with basal secretory activity. Thus, three-dimensional HEMOCOLLAGENE® foams present an in vitro model that mimics the MSC niche with the capacity to support viable and quiescent MSCs within a low stiffness collagen I scaffold simulating Wharton's jelly. These results suggest that haemostatic foam may be a useful and versatile carrier for MSC transplantation for regenerative medicine applications.

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