Do different probing depths exhibit striking differences in microbial profiles?

Abstract

Aim

To perform a thorough characterization of the subgingival microbiota of shallow, moderate and deep sites in subjects with chronic periodontitis (ChP)

Material and methods

Subgingival samples were collected from subjects with ChP (n=3/category of probing depth: ≤3, 4-6 and ≥7 mm) and periodontal health (PH). Samples were individually analyzed by high-throughput sequencing and the sequences were analyzed using mothur and R packages

Results

Nine subjects with ChP and seven with PH were included and 101 samples were evaluated. Thirteen phyla, 118 genera and 211 OTUs were detected. Taxa from Chloroflexi and Spirochaetes phyla were associated with initial stages of disease. Fretibacterium, Eubacterium[XI][G-6], Desulfobulbus, Peptostreptococcaceae[XI][G-1] and [G-3], Bacteroidetes[G-3], Bacteroidaceae[G-1] genera and Filifactor alocis, Fretibacterium fastidiosum, Johnsonella sp HOT166, Peptostreptococcaceae[XIII][G-1] HOT113, Porphyromonas endodontalis and Treponema sp. HOT258, which are not conventionally associated with disease, increased with the deepening of the pockets and/or were elevated in ChP; while Streptococcus, Corynebacterium and Bergeyella genera were associated with PH (p<0.05)

Conclusion

Striking differences were observed between the microbiota of shallow and moderate/deep sites in ChP subjects. Differences between shallow sites in PH and ChP were also observed. The characterized microbiota included known oral microorganisms and newly identified periodontal taxa, some of them not-yet cultivated.

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