Lytic polysaccharide monooxygenases (LPMOs) are oxidative biocatalysts important for biofuel development. LPMOs use molecular oxygen and an external electron donor to cleave chemically recalcitrant polysaccharide. Because of LPMOs important role in carbohydrate turnover, interest in their mechanism of action and identification of their redox partners has recently surged. Current knowledge of the electron donor partners in LPMO-catalyzed reactions solely relies on observations of LPMO co-secretory proteins and on conjecture about the molecules that may act as LPMO-associated reductants. Here, using direct protein-protein interaction studies of a fungal secretome, we sought to identify LPMOs endogenous redox partner. To this end, we searched the genome and secretome of the fungus Botrytis cinerea for the presence of LPMOs belonging to auxiliary activity family 9 (AA9) superfamily and identified BcLPMO9C as the predominant secreted LPMO. Using pull-down, label-transfer, and other biochemical assays, we then scrutinized the fungal secretome for a probable redox partner of BcLPMO9C and identified cellobiose dehydrogenase_53425 (CDH_53425) as its electron donor partner. Their interaction was validated with experimental strategies such as competition ELISA, surface plasmon resonance and label-transfer studies. Further, using X-ray diffraction and SEM analyses, we established that BcLPMO9C and CDH_53425 are redox partners in degrading complex biomass. Overall, our study provides for the first time a rational approach for secretome analysis to identify an endogenous redox partner of LPMO. This advancement in our knowledge on the molecular mechanisms of the LPMO-CDH interaction will provide insights into improving cellulolytic enzyme cocktails used in the biofuels industry.
from # All Medicine by Alexandros G. Sfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2wyvtkQ
via IFTTT
Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
Αρχειοθήκη ιστολογίου
-
►
2023
(272)
- ► Φεβρουαρίου (141)
- ► Ιανουαρίου (131)
-
►
2022
(2066)
- ► Δεκεμβρίου (80)
- ► Σεπτεμβρίου (170)
- ► Φεβρουαρίου (190)
- ► Ιανουαρίου (203)
-
►
2021
(7399)
- ► Δεκεμβρίου (186)
- ► Σεπτεμβρίου (472)
- ► Φεβρουαρίου (851)
-
►
2020
(2517)
- ► Δεκεμβρίου (792)
- ► Σεπτεμβρίου (21)
- ► Φεβρουαρίου (28)
-
►
2019
(12076)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (4765)
- ► Ιανουαρίου (5155)
-
►
2018
(3144)
- ► Δεκεμβρίου (3144)
-
▼
2017
(66935)
- ► Δεκεμβρίου (1995)
- ► Σεπτεμβρίου (12424)
-
▼
Αυγούστου
(11466)
-
▼
Αυγ 21
(371)
- Effects of Autologous Cytokine-Induced Killer Cell...
- In Vivo and In Vitro Effects of ATM/ATR Signaling ...
- Clinical Effects of CpG-Based Treatment on the Eff...
- Selected Literature Watch
- Automatic affective responses towards the bed in p...
- Inflammation, Stem Cells, and the Aging Hypothalamus
- Deficient Data Dissemination Does Damage
- Dietary Proteins Rapidly Altered the Microbial Com...
- Exploring Furnas-McGregor paradox: describing norm...
- Reconstruction of full-thickness cheek defect with...
- Anatomy of superficial inferior epigastric vessels...
- The clinical impact of bedside fiberoptic laryngos...
- Semicircular canal dehiscence among idiopathic int...
- In response to “in reference to is esophagoscopy n...
- Sonographic differentiation between lymphatic and ...
- Intraosseous mucoepidermoid carcinoma: Outcome review
- Gastrostomy in the era of minimally invasive head ...
- A case-control evaluation of fungiform papillae de...
- Persistent respiratory effort after adenotonsillec...
- What is the role of imaging in the evaluation of t...
- Is acupuncture an effective complementary tool wit...
- Effect of postoperative radiotherapy in pT1pN1cM0 ...
- Expiratory muscle strength training for radiation-...
- Volumetric analysis of olfactory neuroblastoma sku...
- In response to letter to the editor to Does mutati...
- A physician's story about pushing against the medi...
- A typical presentation of a hepatocellular carcino...
- Coliform
- Novel PCR primers to diagnose visceral leishmanias...
- Systematic and quantitative assessment of hydrogen...
- The 2017 Annual Meeting of the International Genet...
- Asthma diagnosis in a child and cessation of smoki...
- Associations among personal care product use patte...
- Exposure of children to extremely low frequency ma...
- Non-smoking pregnant women and their fetuses are e...
- Uncertainty in the estimated risk of lung function...
- Sun exposure and 25-hydroxyvitamin D3 levels in a ...
- Radiofrequency-electromagnetic field exposures in ...
- Nocturnal blood pressure fluctuations measured by ...
- 'I had throat cancer and survived' - Independent.ie
- Targeting thyroid cancer with acid-triggered relea...
- Isolation and characterization of string-forming f...
- Properties of a family 56 carbohydrate-binding mod...
- Identification of an endogenous redox partner for ...
- Spermine inhibits Vibrio cholerae biofilm formatio...
- Recently duplicated plant heterotrimeric G{alpha} ...
- At 24, This Boy Is The Youngest Patient To Have Be...
- Postnatal hypoxia evokes persistent changes within...
- Photoacoustic imaging for in vivo quantification o...
- Nicotinamide riboside, a form of vitamin B3, prote...
- Gab2 mediates hepatocellular carcinogenesis by int...
- Activation of liver X receptor suppresses angiogen...
- Association of increased gait variability while du...
- PI-273, a substrate-competitive, specific small mo...
- Monocarboxylate transporter MCT1 promotes tumor me...
- STRAP promotes stemness of human colorectal cancer...
- Different engagement of TLR2 and TLR4 in Porphyrom...
- Comparing depth-dependent curing radiant exposure ...
- Ki-67 protein predicts survival in oral squamous c...
- Longitudinal impact of clinical and socioenvironme...
- Radioiodine therapy for thyroid cancer doesn't up ...
- Pulsating sounds in head may be pulsatile tinnitus...
- Emergency contraception in a public health emergen...
- Relationship of strategies for emotion cognitive a...
- Addressing the challenges of diagnostic delay and ...
- Strengthening research and development activities ...
- Network creation to support the international coll...
- Safe space: An effective option to ensure normalcy...
- Reactome analysis of Zika virus genes: Implication...
- Involving fathers in achieving gender equality thr...
- How can we eradicate informal payments for health ...
- The challenges of antenna modification in medical ...
- INSPIRE: A comprehensive package to reduce violenc...
- Taking major strides in dengue vaccine research: W...
- Liver problem in zika virus infection: Possibility?
- Census: A systematic and comprehensive tool to add...
- What can we learn from Google Map base GIS system ...
- Legal protection for women in Yemen: A sorry state
- Kidnapping Detection and Recognition in Previous U...
- Gut Microbial Influences on the Mammalian Intestin...
- Improved Finite Beam Element Method to Analyze the...
- Large Subcapsular Splenic Hematoma with a Large Pa...
- Charges and Electromagnetic Radiation as Topologic...
- Locality-Based Visual Outlier Detection Algorithm ...
- On the Characterization and Enumeration of Some Ge...
- Leaving the labour market later in life: how does ...
- Cabozantinib Effective as Salvage Therapy in Thyro...
- Concentration, sources and risk assessment of PAHs...
- Postepy Hig Med Dosw 2017; 71:703-712 "Immune diso...
- Postepy Hig Med Dosw 2017; 71:713-726 "The role of...
- Brachytherapy attains abscopal effects when combin...
- Salvage brachytherapy for recurrent prostate cance...
- Performance of ovarian cyst fluid fine-needle aspi...
- Identification of distinct cytomorphologic feature...
- A pattern-based risk-stratification scheme for sal...
- Dibenzoylmethane Protects Against CCl4-Induced Acu...
- Sonography in diagnosis of adhesive capsulitis of ...
- Systematic and quantitative assessment of hydrogen...
- Optimum ridge-to-furrow ratio in ridge-furrow mulc...
- Regeneration and reuse waste from an edible oil re...
-
▼
Αυγ 21
(371)
Ετικέτες
Πληροφορίες
Δευτέρα 21 Αυγούστου 2017
Identification of an endogenous redox partner for lytic polysaccharide monooxygenase-based oxidative cleavage of polysaccharides [Bioenergetics]
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Αναζήτηση αυτού του ιστολογίου
! # Ola via Alexandros G.Sfakianakis on Inoreader
-
Publication date: 1 May 2019 Source: Talanta, Volume 196 Author(s): Ruiqing Long, Te Li, Chaoying Tong, Lihui Wu, Shuyun Shi Abstract...
-
Oral Cancer Rapid Test Kit Market Rugged Expansion Foreseen by 2024 MilTech Oral cancer is one of the largest group of cancers ...
-
Related Articles SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4...
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,