Comparison of antibiotic resistance phenotypes in laboratory strains and clinical isolates of Staphylococcus aureus , Salmonella Typhimurium, and Klebsiella pneumoniae

Abstract

This study was designed to evaluate the antibiotic resistance phenotypes in wild-type Staphylococcus aureus (WT-SA), oxacillin-induced S. aureus (OI-SA), clinically-acquired antibiotic-resistant S. aureus (CA-SA), wild-type Salmonella Typhimurium (WT-ST), ciprofloxacin-induced S. Typhimurium (CI-ST), clinically-acquired antibiotic-resistant S. Typhimurium (CA-ST), wild-type Klebsiella pneumoniae (WT-KP), ciprofloxacin-induced K. pneumoniae (CI-KP), and clinically-acquired antibiotic-resistant K. pneumoniae (CA-KP). The resistance of WT-SA, WT-ST, and WT-KP to ampicillin, ceftazidime, and cephalotin, penicillin was increased after induction by oxacillin OI-SA, ciprofloxacin CI-ST, and ciprofloxacin CI-KP, respectively. The highest β-lactamase activities were 12 and 36 μmol/min/ml, respectively, for CA-ST and CA-KP. The EtBr residues remained high in S. Typhimurium (>80%) and K. pneumoniae (>90%) when treated with CCCP. The distinct FT–IR spectra were observed in protein region (1700–1500 cm⁻¹) and carbohydrate region (1200–900 cm⁻¹). This study would provide useful information for better understating of specific resistance mechanisms in association with β-lactamase and efflux pump activities.

from # All Medicine by Alexandros G. Sfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2uQufxE
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