miR-21-5p is associated with the regulation of estradiol benzoate and oxytocin induced primary dysmenorrhea in rat uterus: a bioinformatic study

Abstract

To determine whether miRNAs, particularly miR-21-5p, are associated with primary dysmenorrhea (PD) and to elucidate the in-depth molecular mechanisms underlying PD regulation, estradiol benzoate and oxytocin were used to induce PD syndrome in rats followed by miRNA microarray and mRNA profiling of the rat uteruses. Additionally, the expression level of miR-21-5p in the uterus was measured by a real time RT-PCR approach. The differentially expressed mRNAs in the uterus of PD syndrome rats were then bioinformatically analyzed by ClueGo to be enriched in specific biological pathways. The targets of miRNAs in the pathways were predicted by TargetScan. Consequently, the expression levels of 13 miRNAs are significantly changed in the uterus of PD syndrome rats, of which 7 are up-regulated (including miR-21-5p) and 6 are down-regulated. Further, 682 mRNAs were found to be differentially expressed, with 267 and 415 are up- and down-regulated, respectively. These genes are significantly enriched in a series of biological pathways closely relevant to miR-21-5p function and uterine smooth muscle (USM) contraction. Our data, for the first time, disclose that miR-21-5p, as well as some other miRNAs, is associated with PD regulation through the involvement of USM contractability.

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