An in vitro study of the long non-coding RNA TUG1 in tongue squamous cell carcinoma

Abstract

Background

This study sought to study the expression of the long non-coding RNA (lncRNA)taurine-upregulated gene 1 (TUG1) in tongue squamous cell carcinoma (TSCC) and reveal its possible function.

Methods

qRT-PCR was used to evaluate 27 samples of fresh TSCC tissues and adjacent normal tongue tissues. siRNA technology was employed to down-regulate TUG1 expression in CAL-27 and SCC-9 cell lines.The3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was utilized to assess cell proliferation ability; apoptosis and cell cycle phases were analysed via flow cytometry.

Results

qRT-PCR findings indicated that the lncRNA TUG1 was up-regulated in TSCC tissues compared with adjacent normal tongue tissues(P<0.05). After TUG1 expression was down-regulated using siRNA technology, cell proliferation was significantly inhibited (P<0.05), and the number of cells in S phase was reduced(P<0.05).

Conclusion

The lncRNA TUG1 may represent a potential oncogene in TSCC.

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