The Effect of Influenza Virus on The Metabolism of Peripheral Blood Mononuclear Cells with Metabolomics Approach

alexandrossfakianakis shared this article with you from Inoreader The Effect of Influenza Virus on The Metabolism of Peripheral Blood Mononuclear Cells with Metabolomics Approach Via Journal of Medical Virology Abstract Respiratory viruses led to many deaths and hospitalization per year in the world. Influenza virus is one of the most important respiratory viruses. Recently, the metabolic studies in viral infections have been widely considered by scientists. Metabolomics states the metabolites present in a living organism under certain conditions. In this study, peripheral blood mononuclear cells were spinoculated using virus produced by the Madin-Darby Canine Kidney cell culture system and cells were harvested following spinoculation by influenza virus. Isolation of peripheral blood mononuclear cells was performed by Ficoll-Paque density gradient centrifugation. Metabolites were extracted using organic and Water approaches. Metabolic profiling was performed by a non-targeted using LC-MS/MS technique. Multivariate analysis methods used to determine the main variables. the metabolic pathways involved were determined using databases. Results of present study showed change in biosynthesis pathways such as lipids, polyamines, catecholamines and vitamins. Findings also showed that it is possible to explain the process of inflammation caused by the influenza virus by studying the metabolism of immune cells. This article is protected by copyright. All rights reserved. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Perceptions of NZ orthodontists and periodontists on the management of gingival recession in orthodontic patients

alexandrossfakianakis shared this article with you from Inoreader Perceptions of NZ orthodontists and periodontists on the management of gingival recession in orthodontic patients Via Australian Dental Journal ABSTRACT Background This study aimed to investigate the perceptions and opinions of orthodontists and periodontists on the management of gingival recession in orthodontic patients. Methods An online survey was sent to 29 periodontists and 80 orthodontists registered and currently practising in New Zealand. All participants answered questions about the timing and clinical indications of mucogingival surgeries in orthodontic patients diagnosed with mucogingival deformities. Results Most periodontists and orthodontists believed that gingival grafts should ideally be performed after orthodontic treatment. In clinical practice, 40% of periodontists indicated that they would receive referrals after completion of orthodontic treatment. However, 29.6% of orthodontists indicated that they would refer to a periodontist before orthodontic treatment in clinical practice. The most crucial factor that affected periodontists' decision making was 'evidence-based guidelines' (35.0%), followed by 'clinical experience' (30.0%) and 'patient concerns' (15.0%). All four factors of 'gingival phenotype', 'presence of gingival recession', 'amount of keratinised tissue' and 'planning specific tooth movements' were equally considered by orthodontists regarding their decision-making. Conclusions The majority of the surveyed New Zealand periodontists and orthodontists expressed a belief that the ideal timing for the management of gingival recessions would be after the completion of orthodontic treatment. © 2022 Australian Dental Association. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Identification of non-synonymous variations in ROBO1 and GATA5 genes in a family with bicuspid aortic valve disease

alexandrossfakianakis shared this article with you from Inoreader Identification of non-synonymous variations in <i>ROBO1</i> and <i>GATA5</i> genes in a family with bicuspid aortic valve disease Via Journal of Human Genetics - Issue - nature.com science feeds Journal of Human Genetics, Published online: 09 May 2022; doi:10.1038/s10038-022-01036-x Identification of non-synonymous variations in ROBO1 and GATA5 genes in a family with bicuspid aortic valve disease View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Avoidance of cutaneous adverse drug reactions induced by antiepileptic drugs based on pharmacogenomics

alexandrossfakianakis shared this article with you from Inoreader Avoidance of cutaneous adverse drug reactions induced by antiepileptic drugs based on pharmacogenomics Via Journal of Human Genetics - Issue - nature.com science feeds Journal of Human Genetics, Published online: 09 May 2022; doi:10.1038/s10038-022-01040-1 Avoidance of cutaneous adverse drug reactions induced by antiepileptic drugs based on pharmacogenomics View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Synergistic antibacterial effects of low-intensity ultrasound and peptide LCMHC against Staphylococcus aureus

alexandrossfakianakis shared this article with you from Inoreader Synergistic antibacterial effects of low-intensity ultrasound and peptide LCMHC against <em>Staphylococcus aureus</em> Via ScienceDirect Publication: International Journal of Food Microbiology Publication date: Available online 9 May 2022 Source: International Journal of Food Microbiology Author(s): Zijin Yuan, Jude Juventus Aweya, Jian Li, Zhongyan Wang, Shiying Huang, Mingjing Zheng, Linfan Shi, Shanggui Deng, Shen Yang View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Particulate matter (PM10) induces in vitro activation of human neutrophils, and lung histopathological alterations in a mouse model

alexandrossfakianakis shared this article with you from Inoreader Particulate matter (PM10) induces in vitro activation of human neutrophils, and lung histopathological alterations in a mouse model Via Earth and environmental sciences : nature.com subject feeds View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Hypokalaemia associated with hydrochlorothiazide used in the treatment of hypertension in NHANES 1999–2018

alexandrossfakianakis shared this article with you from Inoreader Hypokalaemia associated with hydrochlorothiazide used in the treatment of hypertension in NHANES 1999–2018 Via Journal of Human Hypertension - Issue - nature.com science feeds Journal of Human Hypertension, Published online: 06 May 2022; doi:10.1038/s41371-022-00704-x Hypokalaemia associated with hydrochlorothiazide used in the treatment of hypertension in NHANES 1999–2018 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Estimation of the COVID‐19 Mean Incubation Time: Systematic Review, Meta‐analysis, and Sensitivity Analysis

alexandrossfakianakis shared this article with you from Inoreader Estimation of the COVID‐19 Mean Incubation Time: Systematic Review, Meta‐analysis, and Sensitivity Analysis Via Journal of Medical Virology Abstract Objectives Providing sensible estimates of the mean incubation time for COVID-19 is important yet complex. This study aims to provide sensible estimates of the mean incubation time of COVID-19 by capitalizing on available estimates reported in the literature and exploring different ways to accommodate heterogeneity involved in the reported studies. Methods Online databases between January 1 of 2020 and May 20 of 2021 are first searched to obtain estimates of the mean incubation time of COVID-19 from available studies, and meta-analyses are then conducted to generate synthetic estimates. Heterogeneity of the studies is examined via the use of Cochran's Q statistic and Higgin's & Thompson's I2 statistic, and subgroup analyses are conducted using mixed effects models. The publication bias issue is assessed using the funnel plot and Egger's test. Results Using all those reported mean incubation estimates for COVID-19, the mean incubation time is estimated to be 6.43 days with a 95% confidence interval (CI) (5.90, 6.96), and using all those reported mean incubation estimates together with those transformed median incubation estimates, the estimated mean incubation time is 6.07 days with a 95% CI (5.70,6.45). Conclusions The reported estimates of the mean incubation time of COVID-19 vary considerably due to multiple reasons, including heterogeneity and publication bias. To alleviate these issues, we take different angles to provide a sensible estimate of the mean incubation time of COVID-19. Our analyses show that the mean incubation time of COVID-19 between January 1 of 2020 and May 20 of 2021 ranges from 5.68 days to 8.30 days. This article is protected by copyright. All rights reserved. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Use of Saliva and RT‐PCR Screening for SARS‐CoV‐2 Variants of Concern: Surveillance and Monitoring

alexandrossfakianakis shared this article with you from Inoreader Use of Saliva and RT‐PCR Screening for SARS‐CoV‐2 Variants of Concern: Surveillance and Monitoring Via Journal of Medical Virology ABSTRACT Genomic surveillance has been applied since the beginning of the COVID-19 pandemic to track the spread of virus, leading to characterization of multiple SARS-CoV-2 variants, including Variants of Concern (VOC). Although sequencing is the standard method, rapid molecular test for screening and surveillance of VOC is considered for detection. Furthermore, using alternative saliva as specimen collection, facilitates the implementation of a less invasive, self-collected sample. In this study, we applied a combinatory strategy of saliva collection and RT-PCR for SARS-CoV-2 VOC detection. Saliva samples from patients attending at a tertiary hospital with suspected COVID-19 were collected and SARS-CoV-2 RNA was detected using SARS-CoV-2 RT-qPCR reagent kit (PerkinElmer). Positive saliva samples were screened for SARS-CoV-2 VOC with previously described RT-PCR for Alpha, Beta and Gamma variants. Saliva samples were positive in 171 (53%) of 324 tested. A total of 108 (74%) from positive samples were also positive for VOC by RT-PCR screening. Those samples were found between January and August 2021. This approach allowed us to successfully use an alternative and complementary tool to genomic surveillance to monitoring the circulation of SARS-CoV-2 VOC in the studied population. This article is protected by copyright. All rights reserved. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Glibenclamide promoted functional recovery following sciatic nerve injury

alexandrossfakianakis shared this article with you from Inoreader Glibenclamide promoted functional recovery following sciatic nerve injury in male Wistar rats Via Fundamental & Clinical Pharmacology Abstract The impact of peripheral nerve damage on a patient's quality of life is severe. The most frequent peripheral nerve crush damage is a sciatic nerve injury. Previous research has shown that glibenclamide (GB) has neuroprotective properties in a variety of oxidative stress-related disorders, including Alzheimer and Parkinson. The goal of this study was to see how GB affected nerve regeneration and improved function of the sciatic nerve in a rat model following a crush injury. We evaluated motor function, sensory recovery, gene expression, and histomorphometry following damage at different time points. Additionally, we assessed atrophy in the gastrocnemius muscle using histology and mass ratio analyses. Our results suggest that 2-, 4-, 6-, and 8-weeks following glibenclamide therapy, promotes the recovery of motor and sensory function in the injured site. Following glibenclamid injection, the mRNA levels of neurotrophic factors (NGF and BDNF) are raised. According to histomorphometry assessment, glibenclamide injection also increased the number of myelinated fibers while decreasing their thickness. These results showed that glibenclamide therapy by decreasing the proinflammatory and oxidant factors may enhance the nerve regeneration. It is clear that more research is needed to confirm these findings. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Loss of dimethylated H3K27 (H3K27me2) expression is not a specific marker of malignant peripheral nerve sheath tumor (MPNST): An immunohistochemical study of 137 cases, with emphasis on MPNST and melanocytic tumors

alexandrossfakianakis shared this article with you from Inoreader Loss of dimethylated H3K27 (H3K27me2) expression is not a specific marker of malignant peripheral nerve sheath tumor (MPNST): An immunohistochemical study of 137 cases, with emphasis on MPNST and melanocytic tumors Via Annals of Diagnostic Pathology Publication date: Available online 6 May 2022 Source: Annals of Diagnostic Pathology Author(s): Judith Jebastin Thangaiah, Brooke E. Westling, Anja C. Roden, Caterina Giannini, Michael Tetzlaff, Woo Cheal Cho, Andrew L. Folpe View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

The anti-tumor effect of proteasome inhibitor MG132 for human adenoid cystic carcinoma: correlate with the emerging role of Nrf2/Keap1 signaling pathway

alexandrossfakianakis shared this article with you from Inoreader The anti-tumor effect of proteasome inhibitor MG132 for human adenoid cystic carcinoma: correlate with the emerging role of Nrf2/Keap1 signaling pathway Via Head & Face Medicine - Latest Articles Adenoid cystic carcinoma (ACC) is one of the most common malignant salivary gland tumors. Moreover, the unique biological characteristics and complex structures of ACC contribute to its poor survival rates. Re... View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Loss of KDM4B impairs osteogenic differentiation of OMSCs and promotes oral bone aging

alexandrossfakianakis shared this article with you from Inoreader Loss of KDM4B impairs osteogenic differentiation of OMSCs and promotes oral bone aging Via International Journal of Oral Science - Issue International Journal of Oral Science, Published online: 07 May 2022; doi:10.1038/s41368-022-00175-3 Loss of KDM4B impairs osteogenic differentiation of OMSCs and promotes oral bone aging View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.