Meta-analysis of the diagnostic value of serum, plasma and urine neutrophil gelatinase-associated lipocalin for the detection of acute kidney injury in patients with sepsis

xlomafota13 shared this article with you from Inoreader Meta-analysis of the diagnostic value of serum, plasma and urine neutrophil gelatinase-associated lipocalin for the detection of acute kidney injury in patients with sepsis Via Experimental and therapeutic medicine Exp Ther Med. 2021 Apr;21(4):386. doi: 10.3892/etm.2021.9817. Epub 2021 Feb 23. ABSTRACT The objective of the present study was to assess the diagnostic value of urine, serum and plasma neutrophil gelatinase-associated lipocalin (NGAL) for the early diagnosis of acute kidney injury (AKI) among patients with suspected sepsis. Therefore, a meta-analysis was carried out to evaluate diagnostic accuracy data from the literature regarding the diagnosis of AKI in patients with sepsis. Electronic databases were systematically searched for relevant studies and quality assessment was conducted using the Quality Assessment for Diagnostic Accuracy Studies 2 tool. A summary receiver operating characteristic curve analysis was performed, and several parameters including sensitivity, specificity, diagnosis odds ratio (DOR) and area under the curve (AUC) were calculated to evaluate the diagnostic performance of urine, serum and plasma NGAL. Meta-regre ssion, sensitivity and subgroup analysis were also conducted to identify the source of heterogeneity in the eligible studies. In total, 28 studies were included. The pooled sensitivities for urine, serum and plasma NGAL were 0.87, 0.83 and 0.80, respectively. Pooled specificity was 0.84, 0.79 and 0.74. The DORs were 35, 18 and 11, respectively. The AUC for urine, serum and plasma NGAL were 0.92, 0.87 and 0.84, respectively. Urine NGAL presented superior performance for the diagnosis of AKI with the highest AUC and other diagnostic accuracy values, compared with serum and plasma NGAL. Further studies are needed to clarify the controversial issue between the usefulness of serum and plasma NGAL. PMID:33680108 | PMC :PMC7918111 | DOI:10.3892/etm.2021.9817 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Cervical malignant mixed mesonephric tumour: A case report with local recurrence after six-years and next-generation sequencing analysis with particular reference to the ataxia telangiectasia mutated gene

xlomafota13 shared this article with you from Inoreader Cervical malignant mixed mesonephric tumour: A case report with local recurrence after six-years and next-generation sequencing analysis with particular reference to the ataxia telangiectasia mutated gene Via Experimental and therapeutic medicine Exp Ther Med. 2021 Apr;21(4):394. doi: 10.3892/etm.2021.9825. Epub 2021 Feb 24. ABSTRACT Malignant mixed mesonephric tumours (MMMsT) of the female genital tract are extremely rare, and the majority are located in the wall of the cervix uteri. At present, there are no reports of the molecular characterisation of MMMsT of the female genital tract. Herein, we report the morphological, immunohistochemical and molecular features of this rare malignancy using next-generation sequencing (NGS) analysis. A 58-year-old woman presented with vaginal bleeding. In 2013, she had been diagnosed with a cervical carcinosarcoma of probable mesonephric origin and International Federation of Gynaecology and Obstetrics (FIGO) stage IB that had been treated by total hysterosalpingo-oopherectomy without adjuvant chemo-radiotherapy. Ultrasonography showed a vaginal mass measuring 25 mm in the maximum dimension. Biopsy was performed and showed a biphasic neopla sm composed of adenocarcinoma and sarcoma. Immunohistochemistry showed positive staining for epithelial membrane antigen (EMA), pancytokeratin (MNF116), paired box 8 (PAX-8), β-catenin, cytokeratin 7, cyclin D1, GATA3 and CD10. Androgen receptor positivity was detected in very limited areas. Cytokeratin 20, carcinoembryonic antigen (CEA), oestrogen receptor (ER), progesterone receptor (PR), transcription termination factor 1 (TTF1), Wilm's tumour antigen-1 (WT-1), calretinin and p16 were negative. The immunohistochemical profile was consistent with mesonephric origin. NGS analysis identified a variant of the ataxia-telangiectasia mutated (ATM) gene (p.Phe858Leu; c.2572 T>C; COSM21826). The number of detected allele frequency reads of ATM mutation following clinical relapse was higher, compared to its baseline: 65 vs. 96%. The differential diagnosis of MMMsT includes mesonephric hyperplasia, malignant mixed Mullerian tumour (carcinosarcoma), endometrioid adenocarcin oma and endometrial stromal sarcoma. The clinical significance of the observed ATM variant in the case reported herein is unknown. The present findings need further verification, as the mutation in ATM may result in chemotherapy resistance or conversely, may be exploited for targeted therapies. PMID:33680116 | PMC:PMC7918045 | DOI:10.3892/etm.2021.9825 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Oroxylin A attenuates IL-1β-induced inflammatory reaction via inhibiting the activation of the ERK and PI3K/AKT signaling pathways in osteoarthritis chondrocytes

xlomafota13 shared this article with you from Inoreader Oroxylin A attenuates IL-1β-induced inflammatory reaction via inhibiting the activation of the ERK and PI3K/AKT signaling pathways in osteoarthritis chondrocytes Via Experimental and therapeutic medicine Exp Ther Med. 2021 Apr;21(4):388. doi: 10.3892/etm.2021.9819. Epub 2021 Feb 24. ABSTRACT Osteoarthritis (OA) is characterized by degradation of the articular cartilage, synovium inflammation, subchondral bone sclerosis and osteophyte formation. OA is the most common degenerative joint disorder among the elderly population. In particular, currently available therapeutic strategies, such as non-steroidal anti-inflammatory drugs (NSAIDs) may cause severe side-effects. Therefore, novel candidate targets for OA therapy are urgently needed. Oroxylin A (OrA) is a natural mono-flavonoid that can be extracted from Scutellariae radix. The present study aimed to investigate the potential effects of OrA on interleukin (IL)-1β-induced chondrocytes inflammatory reactions. The current study performed quantitative PCR, western blotting and cell immunofluorescence to evaluate the effect of Oroxylin A in chondrocyte inflammation. The results dem onstrated that OrA significantly attenuated the upregulation of inducible nitric oxide synthase and cyclooxygenase 2 by IL-1β at both protein and mRNA levels. IL-1β-stimulated upregulation of matrix metalloproteinase (MMP)-3 and MMP-13 expression, in addition to disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-4 and ADAMTS-5 expression, were all inhibited by OrA. Treatment with OrA significantly reversed the degradation of type II collagen and aggrecan by IL-1β. Mechanistically, OrA suppressed the IL-1β induced activation of ERK1/2 and PI3K/AKT signaling pathways. In conclusion, these findings suggest that OrA can serve as a potential therapeutic agent for the treatment of OA. PMID:33680110< /a> | PMC:PMC7918508 | DOI:10.3892/etm.2021.9819 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Clinical and molecular characterization of seven patients with Danon disease

xlomafota13 shared this article with you from Inoreader Clinical and molecular characterization of seven patients with Danon disease Via Experimental and therapeutic medicine Exp Ther Med. 2021 Apr;21(4):395. doi: 10.3892/etm.2021.9826. Epub 2021 Feb 24. ABSTRACT Danon disease is an X-linked glycogen storage disease characterized by skeletal myopathy, cardiomyopathy and intellectual impairment. It is caused by a loss-of-function mutation in the lysosome-associated membrane protein-2 (LAMP2) gene. In the present study, exon and boarding intron analysis of 96 cardio disease-associated genes was performed in 770 patients with hypertrophic cardiomyopathy (HCM) using second-generation sequencing. Next, the identified mutations were confirmed in family members of the patients and 300 healthy controls. Detailed clinical, electrocardiographic (ECG) and echocardiographic findings were recorded. A pathogenic mutation in LAMP2 was identified in 7 patients who phenotypically presented with HCM. A total of four patients had a fragmented QRS complex (fQRS) on surface ECG. In addition, two patients presented with ventricu lar preexcitation with a short PR interval. Compared with the patients with protein kinase AMP-activated non-catalytic subunit γ2 syndrome and Fabry disease, the 7 patients with Danon disease presented at an earlier age, had a smaller left atrial size, a thinner maximal left ventricular wall thickness and a lower probability of pacemaker implantation. Compared with 12 sex- and age-matched patients with sarcomere-protein mutations, the 4 patients with Danon disease had a lower left ventricular outflow tract gradient and worse diastolic function. The present study provided a comprehensive comparison of different pathologies presenting with HCM and reported on features of early-onset Danon disease, including the characteristic preexcitation and fQRS on ECG. This may provide valuable information that may be utilized for the early diagnosis and treatment of patients with Danon disease. The present study was registered as a clinical trial with ClinicalTrials.gov (Sep. 2, 2016; registry n o. NCT02888132). PMID:33680117 | PMC:PMC7918051 | DOI:10.3892/etm.2021.9826 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

CENPF promotes papillary thyroid cancer progression by mediating cell proliferation and apoptosis

xlomafota13 shared this article with you from Inoreader CENPF promotes papillary thyroid cancer progression by mediating cell proliferation and apoptosis Via Experimental and therapeutic medicine Exp Ther Med. 2021 Apr;21(4):401. doi: 10.3892/etm.2021.9832. Epub 2021 Feb 24. ABSTRACT Papillary thyroid cancer (PTHCA) accounts for ~85% cases of thyroid cancer and exhibits high incidence. Targeted therapy is an effective method to combat this disease; however, novel therapeutic targets are required. Centromere protein F (CENPF), a member of centromere proteins and a transient kinetochore protein, regulates various cellular processes such as cell migration and mitosis, and its upregulation has been observed in multiple types of cancer, including breast cancer and gastric cancer. However, the potential role of CENPF in PTHCA progression is remains unclear. The results of the current study demonstrated that CENPF expression was enhanced in human PTHCA tissues through IHC assays. Furthermore, the expression of CENPF was correlated with the prognosis and the clinicopathological features, including T stage (P=0.021) and intraglandular d issemination (P=0.042) in patients with PTHCA. CENPF regulated the proliferation, apoptosis and cell cycle of PTHCA cells in vitro, which was confirmed through colony formation, MTT and flow cytometry assays, and affected tumor growth in vivo in mice. In conclusion, the current study reported the involvement of CENPF in PTHCA progression and provided a promising therapeutic target for PTHCA treatment. PMID:33680123 | PMC:PMC7918471 | DOI:10.3892/etm.2021.9832 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Thrombotic microangiopathy led to acute kidney injury in an infant with hemophagocytic lymphohistiocytosis: A case report

xlomafota13 shared this article with you from Inoreader Thrombotic microangiopathy led to acute kidney injury in an infant with hemophagocytic lymphohistiocytosis: A case report Via Experimental and therapeutic medicine Exp Ther Med. 2021 Apr;21(4):396. doi: 10.3892/etm.2021.9827. Epub 2021 Feb 24. ABSTRACT Hemophagocytic lymphohistiocytosis (HLH) is a rare but fatal clinical syndrome frequently complicated by acute kidney injury (AKI) and acute tubular necrosis. Renal thrombotic microangiopathy (TMA) is a specific pathological feature of childhood HLH and few cases have been reported among infants. The present study presents a rare case of HLH with TMA in an infant. A 15-month-old infant with a week-long history of fever was admitted to hospital. The infant presented with AKI and subsequently a reduction in platelet and hemoglobin levels. TMA was diagnosed by kidney biopsy and the clinical, laboratory and bone marrow biopsy findings met the criteria of HLH. Due to a progressive increase in serum creatinine levels, hemodialysis was initiated on the second day following admission. Dexamethasone was administered to treat both the fever and HLH. The pati ent's body temperature returned to a normal range and platelet and hemoglobin levels were stable after 14 days of admission. Renal function stabilized on day 21. The results of genetic testing did not identify any disease-related variations. Childhood HLH is a severe condition and mortality can be reduced by early diagnosis and correct treatment. For patients with HLH and AKI, the possible role of TMA should be considered. Renal biopsy can help to identify the cause of AKI and can be performed when the patient's condition is stable. PMID:33680118 | PMC:PMC7918195 | DOI:10.3892/etm.2021.9827 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Proteomics analysis of the hypothalamus in spontaneously hypertensive rats treated with twirling reinforcing manipulation, twirling reducing manipulation or electroacupuncture

xlomafota13 shared this article with you from Inoreader Proteomics analysis of the hypothalamus in spontaneously hypertensive rats treated with twirling reinforcing manipulation, twirling reducing manipulation or electroacupuncture Via Experimental and therapeutic medicine Exp Ther Med. 2021 Apr;21(4):381. doi: 10.3892/etm.2021.9812. Epub 2021 Feb 19. ABSTRACT Hypertension is one of the primary risk factors for cardiovascular diseases. Numerous proteins serve a critical role in hypertension. Acupuncture has been widely used as a treatment for hypertension in China. The results of the current study suggested that electroacupuncture (EA), twirling reinforcing manipulation (TRFM) and twirling reducing manipulation (TRDM) may be useful in the treatment of hypertension. Additionally, proteome analysis of spontaneously hypertensive rats treated with EA, TRFM and TRDM was performed. There were 117 (EA group), 61 (TRFM group) and 86 (TRDM group) differentially expressed proteins (DEPs) identified in the respective experimental groups compared with the model group. Moreover, parallel reaction monitoring assays were used to validate the reliability of the DEPs. The majority of the results were consistent with prev ious proteomics results, in particular that for expression of neudesin neurotrophic factor (NENF). NENF may potentially represent an antihypertensive drug target. PMID:33680103 | PMC:PMC7918484 | DOI:10.3892/etm.2021.9812 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Effect of Endoscopic Sinus Surgery on Clinical Outcomes in DeltaF508 Cystic Fibrosis Patients

xlomafota13 shared this article with you from Inoreader Effect of Endoscopic Sinus Surgery on Clinical Outcomes in DeltaF508 Cystic Fibrosis Patients Via Clinical Otolaryngology Abstract Objectives Chronic rhinosinusitis (CRS) is prevalent in the Cystic Fibrosis (CF) population. CRS exacerbations in CF are thought to contribute to pulmonary exacerbations. Literature regarding the impact of endoscopic sinus surgery (ESS) is inconclusive. This study examines rates of lung function decline and pulmonary exacerbation in CF patients who have undergone ESS. Design Retrospective review of medical records. Setting Academic Hospital. Participants 40 adult CF patients. Main outcome measures Rate of lung function decline (% predicted Forced Expiratory Volume in 1 second [ppFEV1]), number of pulmonary exacerbations (IV/oral antibiotic therapy +/‐ hospital admission) and total number days hospitalized 2 years post‐operatively was collected. CRS patients undergoing ESS were matched to those without ESS by gender, age, and F508del genotype. Results Forty patients (mean age 37.4, 60% male) were reviewed. No significant difference was found between the surgical group and controls in baseline ppFEV1(72.5% vs. 72.7%, p=0.98), 2‐year pre‐operative number of pulmonary exacerbations (3.05 vs. 1.65, p=0.10), or Lund‐Mackay scores (12.25 vs. 11.55, p=0.71). No significant difference was found in 1‐year (70.5% vs. 72.8%, p=0.84) or 2‐year (70.4% vs. 72.6% p=0.80) post‐operative ppFEV1 and 2‐year post‐operative pulmonary exacerbations (1.7 vs. 1.45, p=0.87). A significant increase was identified in total number days hospitalized post‐operatively (4.85, p=0.02). In the surgical group, no significant difference was identified between preoperative and postoperative ppFEV1, 1 ‐year (‐2.51%, p=0.32) and 2‐years after ESS (‐3.10%, p=0.51), postoperative rate of pulmonary exacerbations (‐1.28, p=0.11), or in total number days hospitalized (3.74, p=0.14). Conclusions In this study, ESS does not appear to significantly improve ppFEV1 or decrease the number of pulmonary exacerbations post‐operatively. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

The Axillary Flap/Trans‐agger Approach as a Supero‐lateral Conduit to the Effective Frontal Recess for Marsupialization of Fronto‐ethmoid Mucocele

xlomafota13 shared this article with you from Inoreader The Axillary Flap/Trans‐agger Approach as a Supero‐lateral Conduit to the Effective Frontal Recess for Marsupialization of Fronto‐ethmoid Mucocele Via Clinical Otolaryngology Abstract Frontal‐orbital‐ethmoid mucocele (henceforth, "the mucocele") is often slow‐growing and insidious, needing watchful observation. But when symptomatic, complete endoscopic marsupialization is the intervention of choice.2,3 The mucocele's free wall, generally seen occupying the frontal recess (FR) in the ethmoid infundibulum/third pass roof, has a convenient transmeatal endoscopic access. However, much of the dependent surface remains hidden supero‐lateral within the bony lateral nasal wall behind the agger nasi, and further posterior into the migrated, intercommunicating ethmoid airspaces along the skull‐base. Failure to address the mucocele completely by marsupializing only its visible, infero‐medial aspect through the transmeatal route alone results in limited sinus drainage, and subsequent recurrence. Here, understanding the concept of "effective FR" (eFR) is essential. The eFR is formed by considering the FR and the migrated ethmoid air� �cells as functional patho‐anatomic compartments for an expanding, space‐occupying lesion like mucocele. The surgical technique described here is based on eFR compartmentalization and the mucocele's preferential path of progress. This ensures complete marsupialization, a wider Draf IIa ventilation/drainage basin, and a safe, co‐planar, superiorly‐placed endoscopic vision ("endo‐vision") across the mucocele and the vascular structures of skull‐base. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

A novel method to objectively assess robotic assistance in laparoscopic colorectal surgery

xlomafota13 shared this article with you from Inoreader A novel method to objectively assess robotic assistance in laparoscopic colorectal surgery Via The International Journal of Medical Robotics and Computer Assisted Surgery Abstract Objective An objective assessment of robotic assistance in colorectal surgery. Background There is a lack of objective evidence behind the claims of superior optics and improved ergonomics with robotics. This study introduces a novel method of assessing how robotics facilitates colorectal surgery. Methods Operative videos of laparoscopic and robotic surgeries were reviewed. Two components were evaluated—scope‐holder performance was assessed using a transparent overlay to partition the video image and provide lines for measurement; assistant instrument performance was measured using a separate matrix of movement episodes and time. Results The views achieved in robotic surgery were superior and less dependent on the assistant. The degree of assistance provided by the robotic system was more than that of the human assistant. Conclusions The use of robotics allows a trained operator to project his skills threefold—as a scope holder, operator and assistant—providing superior visualization and dynamic assistance throughout surgery. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

The Feasibility of Gastroesophageal Manometry for Continuously Evaluating the Degree of Expiratory Effort During Successful Crescendo Phonation

xlomafota13 shared this article with you from Inoreader The Feasibility of Gastroesophageal Manometry for Continuously Evaluating the Degree of Expiratory Effort During Successful Crescendo Phonation Via Voice Publication date: Available online 8 March 2021 Source: Journal of Voice Author(s): Masanori Umatani, Makoto Ogawa, Kiyohito Hosokawa, Chieri Kato, Eri Okajima, Toshihiko Iwahashi, Hidenori Inohara View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.