MicroRNA-92b promotes cell proliferation and invasion in osteosarcoma by directly targeting Dickkopf-related protein 3

xlomafota13 shared this article with you from Inoreader MicroRNA-92b promotes cell proliferation and invasion in osteosarcoma by directly targeting Dickkopf-related protein 3 Via Experimental and therapeutic medicine Exp Ther Med. 2021 Nov;22(5):1258. doi: 10.3892/etm.2021.10693. Epub 2021 Sep 3. ABSTRACT [This retracts the article DOI: 10.3892/etm.2017.5356.]. PMID:34603526 | PMC:PMC8453327 | DOI:10.3892/etm.2021.10693 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Antibodies against thyroid-stimulating hormone receptor cause maternal-neonatal transmission of Graves' Disease

xlomafota13 shared this article with you from Inoreader Antibodies against thyroid-stimulating hormone receptor cause maternal-neonatal transmission of Graves' Disease Via Experimental and therapeutic medicine Exp Ther Med. 2021 Nov;22(5):1253. doi: 10.3892/etm.2021.10688. Epub 2021 Sep 3. ABSTRACT The present study aimed to investigate whether the thyroid-stimulating hormone receptor (TSHR) autoantibodies (Ab) from mothers with Graves' disease (GD) could cause neonatal thyroid disease and the underlying mechanisms of this. An adenovirus expressing the TSHR A-subunit and a control adenovirus expressing β-galactosidase was constructed by Beijing Sino Geno Max Co., Ltd. The sequences were subsequently verified and amplified via PCR. A GD model was established in female BALB/c mice (n=90) by three intramuscular injections of a TSHR-expressing adenovirus (Ad-TSHR). Mice injected with Ad-β-galactosidase served as a sham immunization group. The immunized females were paired with unimmunized males to generate offspring. The serum levels of TSHR-Ab and thyroxine (T4) of mothers and neonates were measured after delivery. Breast milk was collected f rom the stomachs of neonatal mice to determine the TSHR-Ab levels. The positive rate of serum TSHR-Ab (>0.3 IU/l) in the TSHR group was 99% (89/90) and 0% in the sham group. The mother mice in the TSHR group had elevated serum T4 levels and the thyroid pathological features of Graves' hyperthyroidism.GD mice gave birth to smaller newborns with thyroid pathological changes and higher serum levels of TSHR-Ab and T4, compared to the offspring in the sham group. The TSHR-Ab levels in breast milk from the GD mice declined with time. Mice immunized with Ad-TSHR exhibited the clinicopathological features of human GD and give birth to neonates with thyroid disease at birth. PMID:34603521 | PMC:PMC8453324 | DOI:10.3892/etm.2021.10688 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Quercetin suppresses inflammatory cytokine production in rheumatoid arthritis fibroblast-like synoviocytes

xlomafota13 shared this article with you from Inoreader Quercetin suppresses inflammatory cytokine production in rheumatoid arthritis fibroblast-like synoviocytes Via Experimental and therapeutic medicine Exp Ther Med. 2021 Nov;22(5):1260. doi: 10.3892/etm.2021.10695. Epub 2021 Sep 6. ABSTRACT Rheumatoid arthritis (RA) is a chronic, progressive and systemic autoimmune disease mainly characterized by symmetric multijoint synovitis. Quercetin has anti-inflammatory, anti-oxidation and immune regulation activities, and therefore shows high medicinal value. The present study aimed to observe the effect of quercetin on fibroblast-like synoviocytes (FLSs) in RA. Rheumatoid arthritis fibroblast-like synoviocytes (RAFLSs) were pretreated with 50 nmol/l quercetin for 2 h and were then stimulated using TNF-α for 24 h for subsequent experiments. RAFLSs were transfected with short interfering (si)-X-inactive specific transcript (XIST), microRNA (miR)-485 mimic, miR-485 inhibitor or si-PSMB8 or combination. ELISA, PCR and western blotting was used to evaluate the effect of quercetin on RAFLSs treated with TNF-α. It was revealed that quercetin inhib ited the production of inflammatory cytokines and the expression of XIST in RAFLSs induced by TNF-α. Bioinformatics analysis indicated that XIST acted as a sponge for miR-485 and that proteasome subunit β type-8 (PSMB8) was a direct target of miR-485. Moreover, PSMB8 functioned as a suppressor in inflammatory cytokine production of RAFLSs induced by TNF-α. Overall, quercetin was observed to inhibit the production of inflammatory cytokines and the expression of XIST in RAFLSs induced by TNF-α. Moreover, XIST-silencing could suppress the inflammatory reaction by sponging miR-485 in cells treated with TNF-α. Altogether, quercetin could suppress the development of RA in vitro. PMID:34603528 | PMC:PMC8453329 | DOI:10.3892/etm.2021.10695 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

MicroRNA-34a directly targets high-mobility group box 1 and inhibits the cancer cell proliferation, migration and invasion in cutaneous squamous cell carcinoma

xlomafota13 shared this article with you from Inoreader MicroRNA-34a directly targets high-mobility group box 1 and inhibits the cancer cell proliferation, migration and invasion in cutaneous squamous cell carcinoma Via Experimental and therapeutic medicine Exp Ther Med. 2021 Nov;22(5):1257. doi: 10.3892/etm.2021.10692. Epub 2021 Sep 3. ABSTRACT [This retracts the article DOI: 10.3892/etm.2017.5245.]. PMID:34603525 | PMC:PMC8453323 | DOI:10.3892/etm.2021.10692 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

The added value of three-dimensional ultrasonography in uterine pathology

xlomafota13 shared this article with you from Inoreader The added value of three-dimensional ultrasonography in uterine pathology Via Experimental and therapeutic medicine Exp Ther Med. 2021 Nov;22(5):1261. doi: 10.3892/etm.2021.10696. Epub 2021 Sep 6. ABSTRACT The rapid development achieved over the last decades in volume rendering of ultrasound data, known as three-dimensional (3D) ultrasound technique, leads to new opportunities for refining the diagnosis in many gynaecologic conditions. The aim of the present study was to evaluate the advantages of 3D ultrasound over two-dimensional (2D) ultrasound in uterine pathology and to establish the optimal time point during the menstrual cycle to perform 3D ultrasound examination in order to achieve the maximum of useful information. A cross-sectional study on 200 patients who underwent gynaecologic 2D and 3D ultrasound examinations was performed. The addition of 3D examination to 2D ultrasound in uterine pathology provided the most useful information concerning: Congenital uterine anomalies, intrauterine devices (IUDs), adenomyosis, and submucous myomas. The findings showed that the 3D ultrasound scan is a useful tool in gynaecology, especially in cases with congenital uterine anomalies, myoma, and IUD. Although initially it was used for research purposes only, recent findings suggest its usefulness in routine ultrasound scan and the possibility of witnessing its introduction as a recommended examination procedure in the foreseeable future. Further research should be conducted in order to establish the sensitivity of 3D ultrasound in the detection of minor endometrial conditions, by correlating the imaging findings with the hysteroscopic results. PMID:34603529 | PMC:PMC8453339 | DOI:10.3892/etm.2021.10696 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Construction of a new cervical anatomically adaptive titanium mesh cage based on measurements of cervical geometry: A morphological and cadaveric study

xlomafota13 shared this article with you from Inoreader Construction of a new cervical anatomically adaptive titanium mesh cage based on measurements of cervical geometry: A morphological and cadaveric study Via Experimental and therapeutic medicine Exp Ther Med. 2021 Nov;22(5):1256. doi: 10.3892/etm.2021.10691. Epub 2021 Sep 3. ABSTRACT Mismatch between the titanium mesh cage and cervical geometries is an important factor that induces subsidence in anterior cervical corpectomy and fusion (ACCF). The aim of the present study was to construct a new quadrate anatomically adaptive titanium mesh cage (AA-TMC) that matches well with the cervical geometries and segmental alignment in one- and two-level ACCF. Computed tomography (CT) scans of 54 individuals were used to measure the cervical endplate geometries. X-rays of 74 young individuals were used to measure the intervertebral body angle (IBA) and intervertebral body height (IBH) of the surgical segments. The AA-TMC was designed based on these measured parameters. A total of 18 cervical cadaveric specimens underwent successive one- and two-level ACCF using the AA-TMC. Postoperatively, the specimens underwent CT scanning to assess the degree of matching of the TMC-endplate interface (TEI), IBA and IBH. A TEI interval <0.5 mm was considered well matching. In the sagittal plane, 93.8% of the inferior endplates were arched, whereas 94.8% of the superior endplates were flat. In the coronal plane, 82.9% of the inferior endplates and 93.8% of the superior endplates were flat. A total of 91.7 and 94.4% of the TEIs were well matched in one- and two-level ACCF, respectively. The postoperative IBA and IBH values were consistent with the values of young individuals. The AA-TMC achieved good matching with cervical geometries and segmental alignment in one- and two-level ACCF, and is proposed for use in ACCF to increase the contact at the TEI and achieve sufficient lordosis restoration. PMID:34603524 | PMC:PMC8453342 | DOI:10.3892/etm.2021.10691 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Biological therapies targeting the type 2 inflammatory pathway in severe asthma (Review)

xlomafota13 shared this article with you from Inoreader Biological therapies targeting the type 2 inflammatory pathway in severe asthma (Review) Via Experimental and therapeutic medicine Exp Ther Med. 2021 Nov;22(5):1263. doi: 10.3892/etm.2021.10698. Epub 2021 Sep 6. ABSTRACT Asthma is a variable chronic respiratory disease characterized by airway inflammation and hyperresponsiveness, bronchoconstriction, and mucus hypersecretion. While most patients with asthma achieve good control of the disease, 5-10% experience severe symptoms and recurrent exacerbation despite the maximal offered therapy with inhaled corticosteroids and long acting bronchodilators. In previous years, novel biological therapies have become available, and various asthma phenotypes that are characterized by specific biomarkers have been identified. Currently approved biological agents target inflammatory molecules of the type 2 inflammatory pathway, and are effective at decreasing the frequency of asthma attacks, controlling symptoms and decreasing use of systemic steroids. The present study reviewed the effectiveness and safety profile of the curren tly approved biological drugs and provided an overview of the assessment of patients with severe asthma who are potentially suitable for biological therapy, in order to help clinicians to select the most appropriate biological agent. PMID:34603531 | PMC:PMC8453334 | DOI:10.3892/etm.2021.10698 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Roles of circular RNAs in the pathogenesis of intervertebral disc degeneration (Review)

xlomafota13 shared this article with you from Inoreader Roles of circular RNAs in the pathogenesis of intervertebral disc degeneration (Review) Via Experimental and therapeutic medicine Exp Ther Med. 2021 Nov;22(5):1221. doi: 10.3892/etm.2021.10655. Epub 2021 Aug 26. ABSTRACT Lower back pain (LBP) is an extremely common symptom and is recognized as a leading contributor to disability and disease burden globally. Intervertebral disc degeneration (IDD) represents a major cause of LBP. However, the molecular mechanisms involved in the pathogenesis of IDD remain unclear, and currently available treatments, including conservative and surgical options, fail to effectively delay, stop or reverse the progression of IDD. Circular RNAs (circRNAs) are a newly discovered group of covalently closed, single-stranded and endogenous non-coding RNAs. A growing body of research has revealed that a number of circRNAs are widely and aberrantly expressed in IDD tissues. Furthermore, they play important roles in the pathogenesis of IDD, including proliferation, apoptosis, senescence, mitophagy, inflammation and extracellular matrix metabol ism, mainly by acting as sponges for microRNAs. The present review aims to summarize the current understanding on the mechanisms of circRNA-mediated regulation in IDD. PMID:34603518 | PMC:PMC8453328 | DOI:10.3892/etm.2021.10655 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Kikuchi-Fujimoto Disease Associated With Mycoplasma Pneumoniae Infection

xlomafota13 shared this article with you from Inoreader Kikuchi-Fujimoto Disease Associated With Mycoplasma Pneumoniae Infection Via ear nose throat Ear Nose Throat J. 2021 Oct 2:1455613211044225. doi: 10.1177/01455613211044225. Online ahead of print. ABSTRACT Kikuchi-Fujimoto disease (KFD) is a self-limited disease that is more common in young Asian women. Typical presentations included fever and cervical lymphadenopathy. The etiology of KFD is unknown, and diagnosis is based mainly on lymph node biopsy. KFD has been reported to be associated with Mycoplasma pneumoniae infection. However, the role of antibiotic t reatment is unclear. We reported 2 cases of KFD associated with Mycoplasma pneumoniae infection and were successfully treated with a macrolide. PMID:34605286 | DOI:10.1177/01455613211044225 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Falx Cerebelli Meningioma: Case Report of a Rarely Occurring Tumor, Management Nuances, and Literature Review

xlomafota13 shared this article with you from Inoreader Falx Cerebelli Meningioma: Case Report of a Rarely Occurring Tumor, Management Nuances, and Literature Review Via leak of cerebrospinal fluid J Neurol Surg Rep. 2021 Sep 29;82(3):e32-e35. doi: 10.1055/s-0041-1735905. eCollection 2021 Jul. ABSTRACT The falx cerebelli is a small crescent fold of dura mater that is attached to the internal occipital crest and projects forward into the posterior cerebellar notch between the cerebellar hemispheres. We report a rare case of a 61-year-old female who presented with a 1-month history of headache and gait instability. Imaging findings were suggestive of a meningioma arising from the falx cerebelli. Complete surgical resection was achieved with a standard posterior fossa midline approach. Duraplasty was performed using animal allograft dura (Duraguard) and additional layers of oxidized cellulose preparation (Surgicel), fibrin sealant, and nonsuturable collagen matrix (Duragen) were utilized to reduce the risk of a cerebrospinal fluid leak. Pathology confirmed a World Health Organization (WHO) grade-I meningioma. Postoperatively, patient with asymptomatic thrombosis of the left transverse/sigmoid sinuses and later with a pseudomeningocele managed with a lumbar drain. To our knowledge, this is the second documented case in the literature. We discuss intraoperative nuances and unique aspects in the postoperative care and management of these patients. PMID:34603931 | PMC:PMC8481004 | DOI:10.1055/s-0041-17359 05 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Correlation of the Neutrophil-to-Lymphocyte Ratio with the Middle Ear Risk Index in Patients with Chronic Otitis Media

xlomafota13 shared this article with you from Inoreader Correlation of the Neutrophil-to-Lymphocyte Ratio with the Middle Ear Risk Index in Patients with Chronic Otitis Media Via Otolaryngology Indian Abstract This study aimed to investigate the neutrophil-to-lymphocyte ratio (NLR) and severity of the chronic otitis media according to middle ear risk index (MERI) scores. The NLR and middle ear risk index were calculated for 210 chronic otitis media (COM) patients retrospectively. NLR compared between COM patients and 159 participants in control group. Pearson correlation analysis was performed for NLR and MERI. In addition, The cut-off value, sensitivity, and specificity for NLR were determined in COM according to the severity of the disease with ROC analysis. The average of NLR in all patients was 1.94 ± 0.89. NLR was calculated as 2.05 ± 1.03 in the patients with COM and 1.79 ± 0.64 in the control group (p = 0.006, 95% CI 0.07–0.44, t test). According to the ROC analysis, the cut-off point was determined as 1.95 for moderate and severe disease in MERI. For the cut of a NLR = 1.95, sensitivity was calculated as 48.5% and the specifici ty 57.1% for moderate and severe COM. MERI score was found significantly higher in patients with postoperative graft perforation (p < 0.001, 95% CI 0.7–1.8). NLR has not related significantly with the MERI score according to linear regression analysis (p = 0.927). NLR was found to be significantly higher in chronic otitis media patients compared to the control group. But NLR was not related to the severity of the disease classified with MERI. The cut-off value, sensitivity and sensitivity rates of NLR obtained was not available for clinically use. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.