Immunohistochemical Grading of Epidural Fibrosis with CD105 Antibody.

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Immunohistochemical Grading of Epidural Fibrosis with CD105 Antibody.

World Neurosurg. 2019 Jan 24;:

Authors: Erdogan U, Tanik C, Tanriverdi O, Gunaldi O, Yilmaz I, Arslanhan A, Ofluoglu AE

Abstract
PURPOSE: Grading of epidural fibrosis is usually performed by histopathological staining in experimental studies. Immunohistochemical methods for grading are not available in routine practice yet. In our study, the effect of tranexamic acid (TXA), a commonly used hemostatic agent in surgical interventions, was evaluated for use against the development of EF with classical histopathological methods and immunohistochemistry using the CD105 antibody, a marker of angiogenesis.
METHODS: Sixteen rats were used. The rats were assigned to two groups, control and TXA group. Laminectomy was performed on the control group. In the treatment group, laminectomy+topical TXA was applied. After sacrificing the rats in the sixth week, histopathological and immunohistochemical examinations and grading of the EF tissue were performed.
RESULTS: Conventional histopathological parameters of fibroblast count, intensity of fibrosis density, and inflammatory cell density, as well as immunohistochemical evaluation with CD105, showed that the grading of EF was comparable between group I and group II (p <0.001).
DISCUSSION: The results of our study have demonstrated that CD105 is compatible with the conventional histopathological grading methods and can be used as a marker to determine the grades of angiogenesis and fibrosis in experimental studies. The results of our study have also shown that TXA, administered locally for hemostasis, reduces the grade of EF in rats following laminectomy. TXA has been observed to cause no toxic effects on neural tissue as it is already commonly used in clinical practice.

PMID: 30685375 [PubMed - as supplied by publisher]

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