Examination of plasma cell-free DNA of glioma patients by whole exome sequencing.

Examination of plasma cell-free DNA of glioma patients by whole exome sequencing.

World Neurosurg. 2019 Jan 28;:

Authors: Sun J, Zhou W, Mao K, He Y, Yao J, Tang Z, Song M, Liu Q, Zhu H, Ju S, Shi J, Shi W

Abstract
OBJECTIVES: To analyze the plasma cell-free DNA (cf-DNA) in glioma patients with high throughout sequencing for a novel non-invasive method for the early diagnosis and management of glioma.
METHODS: six patients with glioma were recruited from the Affiliated Hospital of Nantong University from June 2015 to September 2016. Their plasma samples were tested for Cf-DNA by Whole Exon Sequencing (WES) and mutations were analyzed by bioinformatics.
RESULTS: After filtering the raw sequencing data of Cf-DNA, 33,173 mutations were obtained from 12,462 genes, of which 442 genes and 655 mutation sites were identical to that in the Cosmic database. However, when we compared the Cf-DNA data with the glioma mutated loci in the TCGA database, only 4 mutations matched with the glioma sequences in TCGA, and did not correspond to that of the paired-tumor tissues.
CONCLUSIONS: There were some cancer-related somatic mutations in the Cf-DNA of glioma patients, but no identical mutations were found in the paired solid tumors. Therefore, plasma Cf-DNA mutations may not be a suitable marker for the detection of glioma.

PMID: 30703591 [PubMed - as supplied by publisher]

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