Physicochemical properties, in vitro cytotoxic and genotoxic effects of PM 1.0 and PM 2.5 from Shanghai, China

Abstract

Exposure to ambient particulate matter (PM) links with a variety of respiratory diseases. However, compared with coarse particles (PM₁₀) and fine particles (PM_2.5), submicrometer particles (PM_1.0) may be a more important indicator of human health risks. In this study, the cytotoxic and genotoxic effects of PM_1.0 samples from Shanghai were examined using A549 cells, and compared with the effects of PM_2.5, to better understand the health effects of PM_1.0 in this area. The PM_1.0 and PM_2.5 samples were characterized for morphology, water-soluble inorganic ions, organic and elemental carbon, and metal elements. The cytotoxicity of PMs was measured using cell viability and cell membrane damage assays. The genotoxic effects of PMs were determined using the comet assay, and DNA damage was quantified using olive tail moment (OTM) values. The physicochemical characterization indicated that PM_1.0 was enriched in carbonaceous elements and hazardous metals (Al, Zn, Pb, Mn, Cu, and V), whereas PM_2.5 was more abundant in large, irregular mineral particles. The biological results revealed that both PM_1.0 and PM_2.5 could induce significant cytotoxicity and genotoxicity in A549 cells, and that exposure to PM_1.0 caused more extensive toxic effects than exposure to PM_2.5. The greater cytotoxic effects of PM_1.0 can be attributed to the combined effects of size and chemical composition, whereas the genotoxic effects of PM_1.0 may be mainly associated with chemical species.

from # All Medicine by Alexandros G. Sfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2sE1jfi
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