Purifying Antibodies Using Ion Exchange Chromatography

The stationary phase is made of immobilized ion exchange groups that have the opposite charge as the target component that is to be carried in the mobile phase. Generally, ion exchange experiments are performed in five stages. What is IEC and how is it performed? The first stage of IEC is called equilibration. During this process, the ion exchange stationary phase is equilibrated to ensure that it is operating at the optimized pH and ionic strength conditions to maximize adsorption of the...

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